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Literature summary for 3.5.4.27 extracted from

  • Pomper, B.K.; Vorholt, J.A.; Chistoserdova, L.; Lidstrom, M.E.; Thauer, R.K.
    A methenyl tetrahydromethanopterin cyclohydrolase and a methenyl tetrahydrofolate cyclohydrolase in Methylobacterium extorquens AM1 (1999), Eur. J. Biochem., 261, 475-480.
    View publication on PubMed
Search for more literature for 3.5.4.27

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 N5,N10-methenyl-5,6,7,8-tetrahydromethanopterin
-
 Methylorubrum extorquens

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl presence of salts required, activity in absence of salts is only 15% of that in the presence of salts Methylorubrum extorquens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 2 * 33000, SDS-PAGE Methylorubrum extorquens
67000
-
 non-denaturing PAGE Methylorubrum extorquens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O Methylorubrum extorquens the enzyme might have a catabolic function in the C1-unit metabolism ?
-
 ?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 the enzyme might have a catabolic function in the C1-unit metabolism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Methylorubrum extorquens
-
-
-
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methylorubrum extorquens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
104
-
-
 Methylorubrum extorquens
630
-
-
 Methylorubrum extorquens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
-
 Methylorubrum extorquens N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
-
 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O the enzyme might have a catabolic function in the C1-unit metabolism Methylorubrum extorquens ?
-
 ?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O the enzyme might have a catabolic function in the C1-unit metabolism Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 33000, SDS-PAGE Methylorubrum extorquens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Methylorubrum extorquens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 salts increase heat stability of the enzyme Methylorubrum extorquens
60
-
 10 min, 50% loss of activity in absence of salts. Stable for 25 min in presence of salt, 1 M K2HPO4 Methylorubrum extorquens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Methylorubrum extorquens