Literature summary for 3.5.4.23 extracted from

Kimura, M.; Kamakura, T.; Tao, Q.Z.; Kaneko, I.; Yamaguchi, I.
Cloning of the blasticidin S deaminase from Aspergillus terreus and its use as a selectable marker for Schizosaccharomyces pombe and Pyricularia oryzae (1994), Mol. Gen. Genet., 242, 121-129.

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Activating Compound

Activating Compound	Comment	Organism	Structure
IPTG	inducer	Aspergillus terreus

Application

Application	Comment	Organism
molecular biology	BSD gene will be useful as a new dominant selectable marker for eukaryotes, first sucessful transformation with a drug resistance gene originating from a eukaryote by selecting for detoxification of the drug	Aspergillus terreus

Cloned(Commentary)

Cloned (Comment)	Organism
bsd overexpressed in Escherichia coli BL 21	Aspergillus terreus
bsr gene employed as marker gene for Escherichia coli, Bacillus subtilis, Nicotiana tabacum, Rhizopus niveus and cultured mammalian cells	Bacillus sp. (in: Bacteria)
PCR amplification of the BSD gene, cloned from cDNA and expressed in Escherichia coli, also transformed into Schizosaccharomyces pombe and Pyricularia oryzae, P. oryzae could not be transformed with bsr, but with BSD	Aspergillus terreus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.021	-	blasticidin S	-	Aspergillus terreus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
13470	-	calculated from amino acid sequence	Aspergillus terreus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
blasticidin S + H2O	Bacillus sp. (in: Bacteria)	-	deaminohydroxyblasticidin S + NH3	-	?
blasticidin S + H2O	Aspergillus terreus	-	deaminohydroxyblasticidin S + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Aspergillus terreus	-	S-712 (ATCC 28865)	-
Bacillus sp. (in: Bacteria)	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
blasticidin S + H2O	-	Bacillus sp. (in: Bacteria)	deaminohydroxyblasticidin S + NH3	-	?
blasticidin S + H2O	-	Aspergillus terreus	deaminohydroxyblasticidin S + NH3	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Aspergillus terreus

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Release 2023.1 (January 2023)