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Literature summary for 3.5.4.21 extracted from
- Properties of crystalline creatinine deiminase from Corynebacterium lilium (1980), Agric. Biol. Chem., 44, 1787-1792.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 1 mM, 4.7% inhibition | Corynebacterium glutamicum |  |
| 2,2'-dipyridyl | 1 mM, 4.5% inhibition | Corynebacterium glutamicum | |
| Ag+ | 1 mM, 96.1% inhibition | Corynebacterium glutamicum | |
| Cu2+ | 1 mM, 83.4% inhibition | Corynebacterium glutamicum | |
| EDTA | 1 mM, 9% inhibition | Corynebacterium glutamicum | |
| Hg2+ | 1 mM, 45.2% inhibition | Corynebacterium glutamicum | |
| p-mercuribenzoate | 1 mM, 49.3% inhibition | Corynebacterium glutamicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.27 | - |
creatinine | - |
Corynebacterium glutamicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 195000 | - |
ultracentrifugation | Corynebacterium glutamicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| creatinine + H2O | Corynebacterium glutamicum | - |
N-methylhydantoin + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Corynebacterium glutamicum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, 20 mM phosphate buffer, pH 7, 60% saturated ammonium sulfate, at least 6 months | Corynebacterium glutamicum |
| 5°C, 20 mM phosphate buffer, pH 7, one week | Corynebacterium glutamicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| creatinine + H2O | - |
Corynebacterium glutamicum | N-methylhydantoin + NH3 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Corynebacterium glutamicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
30 min, 13% loss in activity | Corynebacterium glutamicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 10 | - |
Corynebacterium glutamicum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | - |
Corynebacterium glutamicum |
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