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Literature summary for 3.5.4.21 extracted from

  • Kim, J.M.; Shimizu, S.; Yamada, H.
    Cytosine deaminase that hydrolyzes creatinine to N-methylhydantoin in various cytosine deaminase-forming microorganisms (1987), Arch. Microbiol., 147, 58-63.
No PubMed abstract available
Search for more literature for 3.5.4.21

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
260000
-
 gel filtration Pseudomonas putida 77

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
creatinine + H2O Escherichia coli
-
 N-methylhydantoin + NH3
-
 ?
creatinine + H2O Proteus mirabilis
-
 N-methylhydantoin + NH3
-
 ?
creatinine + H2O Pseudomonas chlororaphis
-
 N-methylhydantoin + NH3
-
 ?
creatinine + H2O Pseudomonas cruciviae
-
 N-methylhydantoin + NH3
-
 ?
creatinine + H2O Pseudomonas chlororaphis subsp. aureofaciens
-
 N-methylhydantoin + NH3
-
 ?
creatinine + H2O Pseudomonas putida 77
-
 N-methylhydantoin + NH3
-
 ?
creatinine + H2O Escherichia coli K12 AKU 0005
-
 N-methylhydantoin + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Proteus mirabilis
-
-
-
Pseudomonas chlororaphis
-
-
-
Pseudomonas chlororaphis subsp. aureofaciens
-
-
-
Pseudomonas cruciviae
-
-
-
Pseudomonas putida 77
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0004
-
 cytosine added to culture medium Pseudomonas cruciviae
0.0011
-
 cytosine added to culture medium Proteus mirabilis
0.0013
-
 cytosine added to culture medium Pseudomonas chlororaphis subsp. aureofaciens
0.0014
-
 polypeptone added to culture medium Pseudomonas cruciviae
0.0015
-
 NH4Cl added to culture medium Pseudomonas chlororaphis subsp. aureofaciens
0.0016
-
 NH4Cl added to culture medium Proteus mirabilis
0.0018
-
 polypeptone added to culture medium Escherichia coli
0.0021
-
 NH4Cl added to culture medium Escherichia coli
0.0024
-
 cytosine added to culture medium Escherichia coli
0.0034
-
 NH4Cl added to culture medium Pseudomonas chlororaphis
0.0051
-
 creatinine added to culture medium Proteus mirabilis
0.0056
-
 polypeptone added to culture medium Pseudomonas chlororaphis subsp. aureofaciens
0.0062
-
 polypeptone added to culture medium Proteus mirabilis
0.0064
-
 NH4Cl added to culture medium Pseudomonas putida 77
0.0065
-
 polypeptone added to culture medium Pseudomonas chlororaphis
0.0072
-
 polypeptone added to culture medium Pseudomonas putida 77
0.0092
-
 cytosine added to culture medium Pseudomonas chlororaphis
0.01
-
 creatinine added to culture medium Pseudomonas chlororaphis subsp. aureofaciens
0.0112
-
 creatinine added to culture medium Pseudomonas chlororaphis
0.0216
-
 creatinine added to culture medium Escherichia coli
0.0729
-
 creatinine added to culture medium Pseudomonas putida 77
0.089
-
 cytosine added to culture medium Pseudomonas putida 77

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
creatinine + H2O
-
 Escherichia coli N-methylhydantoin + NH3
-
 ?
creatinine + H2O
-
 Proteus mirabilis N-methylhydantoin + NH3
-
 ?
creatinine + H2O
-
 Pseudomonas chlororaphis N-methylhydantoin + NH3
-
 ?
creatinine + H2O
-
 Pseudomonas cruciviae N-methylhydantoin + NH3
-
 ?
creatinine + H2O
-
 Pseudomonas chlororaphis subsp. aureofaciens N-methylhydantoin + NH3
-
 ?
creatinine + H2O
-
 Pseudomonas putida 77 N-methylhydantoin + NH3
-
 ?
creatinine + H2O
-
 Escherichia coli K12 AKU 0005 N-methylhydantoin + NH3
-
 ?