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Literature summary for 3.5.4.2 extracted from

  • Jankowska, D.A.; Faulwasser, K.; Trautwein-Schult, A.; Cordes, A.; Hoferichter, P.; Klein, C.; Bode, R.; Baronian, K.; Kunze, G.
    Arxula adeninivorans recombinant adenine deaminase and its application in the production of food with low purine content (2013), J. Appl. Microbiol., 115, 1134-1146.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Blastobotrys adeninivorans

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 66% residual activity at 1 mM Blastobotrys adeninivorans
Cd2+ 28% residual activity at 1 mM Blastobotrys adeninivorans
Cu2+ 64% residual activity at 1 mM Blastobotrys adeninivorans
Fe2+ 7% residual activity at 1 mM Blastobotrys adeninivorans
Mn2+ 32% residual activity at 1 mM Blastobotrys adeninivorans
additional information not inhibited by EDTA Blastobotrys adeninivorans
Ni2+ 48% residual activity at 1 mM Blastobotrys adeninivorans
Zn2+ 56% residual activity at 1 mM Blastobotrys adeninivorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
2,6-diaminopurine at pH 7.4 and 40°C Blastobotrys adeninivorans
0.66
-
adenine at pH 7.4 and 40°C Blastobotrys adeninivorans

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Blastobotrys adeninivorans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+, Mo2+, and Ca2+ have either no effect or only a weak effect on enzyme activity Blastobotrys adeninivorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
gel filtration Blastobotrys adeninivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
adenine + H2O Blastobotrys adeninivorans 100% activity hypoxanthine + NH3
-
?
adenine + H2O Blastobotrys adeninivorans LS3 100% activity hypoxanthine + NH3
-
?

Organism

Organism UniProt Comment Textmining
Blastobotrys adeninivorans L8BT48
-
-
Blastobotrys adeninivorans LS3 L8BT48
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration Blastobotrys adeninivorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.88
-
after 5.26fold purification, at pH 7.4 and 40°C Blastobotrys adeninivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-diaminopurine + H2O 73% activity compared to adenine Blastobotrys adeninivorans ?
-
?
2,6-diaminopurine + H2O 73% activity compared to adenine Blastobotrys adeninivorans LS3 ?
-
?
2-amino-6-bromopurine + H2O 109% activity compared to adenine Blastobotrys adeninivorans ?
-
?
2-amino-6-bromopurine + H2O 109% activity compared to adenine Blastobotrys adeninivorans LS3 ?
-
?
6-chloropurine + H2O 119% activity compared to adenine Blastobotrys adeninivorans ?
-
?
6-chloropurine + H2O 119% activity compared to adenine Blastobotrys adeninivorans LS3 ?
-
?
adenine + H2O 100% activity Blastobotrys adeninivorans hypoxanthine + NH3
-
?
adenine + H2O 100% activity Blastobotrys adeninivorans LS3 hypoxanthine + NH3
-
?
additional information no activity with guanine, adenosine, inosine, guanosine, xanthosine, 2-chloroadenine, 2-amino-7-deazapurine, FAD, and AMP Blastobotrys adeninivorans ?
-
?
additional information no activity with guanine, adenosine, inosine, guanosine, xanthosine, 2-chloroadenine, 2-amino-7-deazapurine, FAD, and AMP Blastobotrys adeninivorans LS3 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Blastobotrys adeninivorans

Synonyms

Synonyms Comment Organism
aadA
-
Blastobotrys adeninivorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Blastobotrys adeninivorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 the enzyme loses 35% of its catalytic function in 1 h at 40°C, whereas at 30°C, the activity of the enzyme remains stable. At 50°C, the activity drops within 10 min to zero Blastobotrys adeninivorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.3
-
2,6-diaminopurine at pH 7.4 and 40°C Blastobotrys adeninivorans
20.8
-
adenine at pH 7.4 and 40°C Blastobotrys adeninivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Blastobotrys adeninivorans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 7.5 the enzyme has maximal stability at 30°C for 24 h in HEPES buffer at pHs between 5.5 and 7.5 Blastobotrys adeninivorans