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Literature summary for 3.5.4.17 extracted from

  • Uchida, H.; Narita, Y.; Masuda, A.; Matsui, Y.; Chen, Y.X.; Takahashi, I.; Maeda, S.; Nomura, A.
    Purification and some properties of adenosine (phosphate) deaminase from the liver of the squid Todarodes pacificus (1995), Biosci. Biotechnol. Biochem., 59, 1276-1280.
No PubMed abstract available
Search for more literature for 3.5.4.17

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 2',3'-cAMP pH 4.0, at 25°C Todarodes pacificus
0.14
-
 adenosine 3'-phenylphosphonate pH 4.0, at 25°C Todarodes pacificus
0.27
-
 2'-deoxyadenosine pH 4.0, at 25°C Todarodes pacificus
0.31
-
 adenosine pH 4.0, at 25°C Todarodes pacificus
0.34
-
 3'-AMP pH 4.0, at 25°C Todarodes pacificus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 2 * 60000, SDS-PAGE Todarodes pacificus
140000
-
 gel filtration Todarodes pacificus

Organism

Organism UniProt Comment Textmining
Todarodes pacificus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Todarodes pacificus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
76.3
-
-
 Todarodes pacificus

Storage Stability

Storage Stability Organism
4°C, stable for 2 month Todarodes pacificus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-cAMP + H2O
-
 Todarodes pacificus 2',3'-cIMP + NH3
-
 ?
2'-deoxyadenosine + H2O
-
 Todarodes pacificus 2'-deoxyinosine + NH3
-
 ?
3'-AMP + H2O
-
 Todarodes pacificus 3'-IMP + NH3
-
 ?
5'-AMP + H2O no activity Todarodes pacificus 5'-IMP + NH3
-
 ?
adenosine + H2O
-
 Todarodes pacificus inosine + NH3
-
 ?
adenosine 3'-phenyl phosphonate + H2O
-
 Todarodes pacificus inosine 3'-phenylphosphonate + NH3
-
 ?
ADP + H2O no activity Todarodes pacificus IDP + NH3
-
 ?
ATP + H2O no activity Todarodes pacificus ITP + NH3
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 60000, SDS-PAGE Todarodes pacificus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5 4 deamination of adenosine 3'-phenylphosphonate Todarodes pacificus
4
-
 deamination of 2',3'-cAMP and 3'-AMP Todarodes pacificus
5.5
-
 deamination of adenosine and 2'-deoxyadenosine Todarodes pacificus

pH Range

pH Minimum pH Maximum Comment Organism
2.5 7 pH 2.5: about 55% of maximal activity at pH 2.5 and at pH 7.0 Todarodes pacificus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7 below 37°C, 20 mM phosphate buffer, stable Todarodes pacificus