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Literature summary for 3.5.4.17 extracted from

  • Su, J.C.; Li, C.C.; Ting, C.C.
    A new adenylate deaminase from red marine alga Porphyra crispata (1966), Biochemistry, 5, 536-543.
    View publication on PubMed
Search for more literature for 3.5.4.17

Activating Compound

Activating Compound Comment Organism Structure
Cl- activates the deamination of ATP, NAD+, ADP and 5'-AMP to the extent of 40%, 30%, 30% and 14% Porphyra crispata

Inhibitors

Inhibitors Comment Organism Structure
F- inhibits the deamination of ATP, NAD+, ADP and 5'-AMP to the extent of 90%, 90%, 86% and 80% respectively Porphyra crispata
Mn2+ inhibitor at neutral and alkaline pH. Activation at acidic pH Porphyra crispata
PCMB
-
 Porphyra crispata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.047
-
 ADP
-
 Porphyra crispata
0.047
-
 5'-AMP
-
 Porphyra crispata
0.066
-
 ATP
-
 Porphyra crispata
0.072
-
 NAD+
-
 Porphyra crispata
0.56
-
 adenosine
-
 Porphyra crispata

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 50% of the activation with Ca2+ Porphyra crispata
Ca2+ enhances activity Porphyra crispata
Mg2+ 50% of the activation with Ca2+ Porphyra crispata
Mn2+ activates at acidic pH. At neutral and alkaline pH it acts as inhibitor Porphyra crispata

Organism

Organism UniProt Comment Textmining
Porphyra crispata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Porphyra crispata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Porphyra crispata

Storage Stability

Storage Stability Organism
-10°C, even a very dilute preparation does not lose any activity after storage for 1 month Porphyra crispata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O
-
 Porphyra crispata 5'-IMP + NH3
-
 ?
adenosine + H2O 28% of the activity with 5'-AMP Porphyra crispata inosine + NH3
-
 ?
ADP + H2O 72% of the activity with 5'-AMP Porphyra crispata IDP + NH3
-
 ?
ATP + H2O 69% of the activity with 5'-AMP Porphyra crispata ITP + NH3
-
 ?
NAD+ + H2O 49% of the activity with 5'-AMP Porphyra crispata nicotinamide-hypoxanthine dinucleotide + NH3
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 pH 6.4, 6 min, 50% loss of activity. At pH 5.1 and 7.1, more than 90% loss of activity after 5 min Porphyra crispata
70
-
 6 min, complete loss of activity Porphyra crispata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4 5.8 deamination of NAD+ Porphyra crispata
5.9 6.5 deamination of ADP Porphyra crispata
6
-
 deamination of ATP Porphyra crispata
6.5 7.5 deamination of adenosine Porphyra crispata
6.8
-
 deamination of 5'-AMP Porphyra crispata