Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli B834(DE3) cells | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
selenomethionine-labeled isozyme GCYH-IB, sitting drop vapor diffusion method, using polyethylene glycol 6000 (10-16% (w/v)), LiCl (1-1.4 M), Tris (50 mM, pH 9.0), and Tris-HCl (50 mM, pH 7.0) | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | no activity detected regardless of metal concentration | Bacillus subtilis | |
Cd2+ | no activity detected regardless of metal concentration | Bacillus subtilis | |
Co2+ | 24% residual activity at 0.1 mM | Bacillus subtilis | |
Co3+ | no activity detected regardless of metal concentration | Bacillus subtilis | |
Cu2+ | no activity detected regardless of metal concentration | Bacillus subtilis | |
EDTA | no activity of GCYH-IB is observed in the presence of EDTA | Bacillus subtilis | |
Fe2+ | 75% residual activity at 1 mM | Bacillus subtilis | |
Fe3+ | no activity detected regardless of metal concentration | Bacillus subtilis | |
Mg2+ | 43% residual activity at 0.1 mM | Bacillus subtilis | |
Ni2+ | 9.8% residual activity at 0.1 mM | Bacillus subtilis | |
Zn2+ | 14% residual activity at 0.05 mM | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00987 | - |
GTP | isozyme GCYH-IB, in 100 mM HEPES (pH 8.0), 100 mM KCl, 0.5 mM MnCl2, 1 mM dithiothreitol, temperature not specified in the publication | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required for activity of isozyme GCYH-1B | Bacillus subtilis | |
Mn2+ | required for activity of isozyme GCYH-1B | Neisseria gonorrhoeae | |
Zn2+ | isozyme GCYH-1A is a Zn2+-dependent enzyme, isozyme GCYH-IB is expressed only under Zn2+-limiting conditions | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28568 | - |
4 * 28568, isozyme GCYH-1B, apparent molecular weight estimated from gel filtration | Neisseria gonorrhoeae |
108600 | - |
isozyme GCYH-1B, apparent molecular weight estimated from gel filtration | Neisseria gonorrhoeae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Neisseria gonorrhoeae | Q5F9K6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Bacillus subtilis | formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate | - |
? | |
GTP + H2O | - |
Neisseria gonorrhoeae | formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodecamer | isozyme GCYH-1A | Bacillus subtilis |
homotetramer | 4 * 28568, isozyme GCYH-1B, apparent molecular weight estimated from gel filtration | Neisseria gonorrhoeae |
homotetramer | isozyme GCYH-1B | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
GCYH-IA | isozyme | Bacillus subtilis |
GCYH-IB | isozyme | Bacillus subtilis |
GCYH-IB | isozyme | Neisseria gonorrhoeae |
GTP cyclohydrolase IA | isozyme | Bacillus subtilis |
GTP cyclohydrolase IB | isozyme | Bacillus subtilis |
GTP cyclohydrolase IB | isozyme | Neisseria gonorrhoeae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
GTP | isozyme GCYH-IB, in 100 mM HEPES (pH 8.0), 100 mM KCl, 0.5 mM MnCl2, 1 mM dithiothreitol, temperature not specified in the publication | Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
physiological function | isozyme GCYH-IB functions to allow folate biosynthesis during Zn2+ starvation | Neisseria gonorrhoeae |
physiological function | isozyme GCYH-IB functions to allow folate biosynthesis during Zn2+ starvation, isozyme GCYH-IB functionally replaces GCYH-IA in Bacillus subtilis under zinc-limiting conditions | Bacillus subtilis |