Literature summary for 3.5.4.16 extracted from

Du, J.; Wei, N.; Xu, H.; Ge, Y.; Vasquez-Vivar, J.; Guan, T.; Oldham, K.T.; Pritchard, K.A.; Shi, Y.
Identification and functional characterization of phosphorylation sites on GTP cyclohydrolase I (2009), Arterioscler. Thromb. Vasc. Biol., 29, 2161-2168.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in HEK-293 cells	Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
S130A	phospho-defective mutant with significantly decreased GCH-1 activity	Rattus norvegicus
S72A	phospho-defective mutant with significantly decreased GCH-1 activity	Rattus norvegicus
T103A	phospho-defective mutant with significantly decreased GCH-1 activity	Rattus norvegicus
T231A	mutant with 23% increased GCH-1 activity but reduced GCH-1 nuclear localization and nuclear GCH-1 activity	Rattus norvegicus
T85A	phospho-defective mutant with 67% decreased GCH-1 activity	Rattus norvegicus
T91A	phospho-defective mutant with significantly decreased GCH-1 activity	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Rattus norvegicus	5737	-
nucleus	-	Rattus norvegicus	5634	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30000	-	SDS-PAGE	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	GCH-1 has 8 potential phosphorylation sites (Ser51, Ser72, Thr85, Thr91, Thr103, Ser130, Ser167, and Thr231). Overexpressed rat GCH-1 is phosphorylated at Ser51, Ser167, and Thr231 in HEK-293 cells	Rattus norvegicus

Subunits

Subunits	Comment	Organism
?	x * 30000, SDS-PAGE	Rattus norvegicus

Synonyms

Synonyms	Comment	Organism
GCH-1	-	Rattus norvegicus

General Information

General Information	Comment	Organism
metabolism	GCH-1 is the rate-limiting enzyme for tetrahydrobiopterin synthesis	Rattus norvegicus

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Release 2023.1 (January 2023)