Cloned (Comment) | Organism |
---|---|
expression of DELTA42-hGTP-CH-I in Escherichia coli M15 | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
4.6 mg/ml purified recombinant DELTA42-hGTP-CH-I in 0.1 M potassium phosphate, pH 7.0, 0.02% NaN3, mixed with precipitation solution containing 6% PEG 6000, 150 mM KCl, 100 mM MOPS, pH 7.0, equilibration against precipitant solution, X-ray diffraction strcuture determination and analysis at 3.1 A resolution, modeling | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | essential for activity, coordinated to a His-side chain of the active site | Escherichia coli | |
Zn2+ | essential for activity, coordinated to a His-side chain of the active site, mechanistic role | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | P30793 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant DELTA42-hGTP-CH-I from Escherichia coli M15 | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
GTP + H2O = formate + 7,8-dihydroneopterin 3'-triphosphate | enzyme contains to thiol groups per active site, reaction mechanism | Escherichia coli | |
GTP + H2O = formate + 7,8-dihydroneopterin 3'-triphosphate | The reaction involves hydrolysis of two C-N bonds and isomerization of the pentose unit, the recyclization may be non-enzymic, reaction mechanism | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Escherichia coli | formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate | - |
? | |
GTP + H2O | - |
Homo sapiens | formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | each subunit contains 1 active site | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
GTP-CH-I | - |
Escherichia coli |
GTP-CH-I | - |
Homo sapiens |