BRENDA - Enzyme Database
show all sequences of 3.5.4.13

A bifunctional dCTP deaminase-dUTP nucleotidohydrolase from the hyperthermophilic archaeon Methanocaldococcus jannaschii

Bjornberg, O.; Neuhard, J.; Nyman, P.O.; J. Biol. Chem. 278, 20667-20672 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Methanocaldococcus jannaschii
Inhibitors
Inhibitors
Commentary
Organism
Structure
dTTP
inhibition of deamination reaction
Methanocaldococcus jannaschii
dUTP
inhibition of deamination reaction
Methanocaldococcus jannaschii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
for dUTP, Km-value in millimolar range
Methanocaldococcus jannaschii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
absolutely required
Methanocaldococcus jannaschii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23300
-
x * 23400, deduced from gene sequence, x * 23300, mass spectrometry
Methanocaldococcus jannaschii
23400
-
x * 23400, deduced from gene sequence, x * 23300, mass spectrometry
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Methanocaldococcus jannaschii
bifunctionality of enzyme avoids release of toxic UTP within cells
?
-
Methanocaldococcus jannaschii
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanocaldococcus jannaschii
-
bifunctional enzyme dCTP deaminase-dUTP nucleotidohydrolase
-
Purification (Commentary)
Commentary
Organism
recombinant protein
Methanocaldococcus jannaschii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dCTP + H2O
-
652395
Methanocaldococcus jannaschii
dUTP + NH3
-
652395
Methanocaldococcus jannaschii
?
ddCTP + H2O
deamination at the same rate as dCTP
652395
Methanocaldococcus jannaschii
ddUTP + NH3
-
652395
Methanocaldococcus jannaschii
?
dUTP + 2 H2O
-
652395
Methanocaldococcus jannaschii
dUMP + 2 phosphate
-
652395
Methanocaldococcus jannaschii
?
additional information
bifunctionality of enzyme avoids release of toxic UTP within cells
652395
Methanocaldococcus jannaschii
?
-
652395
Methanocaldococcus jannaschii
?
Subunits
Subunits
Commentary
Organism
?
x * 23400, deduced from gene sequence, x * 23300, mass spectrometry
Methanocaldococcus jannaschii
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.1
-
dTTP
pH 6.8, 25°C
Methanocaldococcus jannaschii
0.5
-
dUTP
-
Methanocaldococcus jannaschii
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Methanocaldococcus jannaschii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
dTTP
inhibition of deamination reaction
Methanocaldococcus jannaschii
dUTP
inhibition of deamination reaction
Methanocaldococcus jannaschii
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.1
-
dTTP
pH 6.8, 25°C
Methanocaldococcus jannaschii
0.5
-
dUTP
-
Methanocaldococcus jannaschii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
for dUTP, Km-value in millimolar range
Methanocaldococcus jannaschii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
absolutely required
Methanocaldococcus jannaschii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23300
-
x * 23400, deduced from gene sequence, x * 23300, mass spectrometry
Methanocaldococcus jannaschii
23400
-
x * 23400, deduced from gene sequence, x * 23300, mass spectrometry
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Methanocaldococcus jannaschii
bifunctionality of enzyme avoids release of toxic UTP within cells
?
-
Methanocaldococcus jannaschii
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant protein
Methanocaldococcus jannaschii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dCTP + H2O
-
652395
Methanocaldococcus jannaschii
dUTP + NH3
-
652395
Methanocaldococcus jannaschii
?
ddCTP + H2O
deamination at the same rate as dCTP
652395
Methanocaldococcus jannaschii
ddUTP + NH3
-
652395
Methanocaldococcus jannaschii
?
dUTP + 2 H2O
-
652395
Methanocaldococcus jannaschii
dUMP + 2 phosphate
-
652395
Methanocaldococcus jannaschii
?
additional information
bifunctionality of enzyme avoids release of toxic UTP within cells
652395
Methanocaldococcus jannaschii
?
-
652395
Methanocaldococcus jannaschii
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 23400, deduced from gene sequence, x * 23300, mass spectrometry
Methanocaldococcus jannaschii
Other publictions for EC 3.5.4.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733177
Oehlenschlaeger
Bacillus halodurans strain C12 ...
Bacillus halodurans, Bacillus halodurans C-125
Appl. Environ. Microbiol.
81
3395-3404
2015
1
-
1
1
-
-
1
-
-
-
-
2
-
12
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684700
Thymark
Mutational analysis of the nuc ...
Escherichia coli
Arch. Biochem. Biophys.
470
20-26
2008
-
-
-
1
4
-
1
-
-
1
-
1
-
3
-
-
-
-
-
-
-
-
2
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
1
4
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
688399
Helt
Mechanism of dTTP inhibition o ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Mol. Biol.
376
554-569
2008
-
-
-
-
-
-
1
1
-
-
-
1
-
6
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686667
Johansson
Regulation of dCTP deaminase f ...
Escherichia coli
FEBS J.
274
4188-4198
2007
-
-
-
1
3
-
1
1
-
1
-
1
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
3
-
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
688732
Zhang
Chloroviruses encode a bifunct ...
Paramecium bursaria Chlorella virus 1
J. Virol.
81
7662-7671
2007
1
-
1
-
-
-
3
2
-
5
1
2
-
4
-
-
1
-
-
-
-
1
4
1
1
1
-
2
2
1
-
-
-
-
-
1
-
1
-
-
-
-
-
3
-
2
-
5
1
2
-
-
-
1
-
-
-
1
4
1
1
1
-
2
2
1
-
-
-
-
-
-
-
-
669375
Johansson
Structures of dCTP deaminase f ...
Escherichia coli
J. Biol. Chem.
280
3051-3059
2005
-
-
-
1
3
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648212
Huffman
Structural basis for recogniti ...
Methanocaldococcus jannaschii
J. Mol. Biol.
331
885-896
2003
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
652395
Bjornberg
A bifunctional dCTP deaminase- ...
Methanocaldococcus jannaschii
J. Biol. Chem.
278
20667-20672
2003
-
-
1
-
-
-
2
1
-
1
2
1
-
4
-
-
1
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
2
2
1
-
1
2
1
-
-
-
1
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209525
Estevenon
An Escherichia coli strain def ...
Escherichia coli
Mol. Gen. Genet.
246
514-518
1995
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209524
Weiss
De novo synthesis of thymidyla ...
Escherichia coli
J. Bacteriol.
176
2194-2199
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209522
Wang
Dcd (dCTP deaminase) gene of E ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
174
5647-5653
1992
-
-
2
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209523
Speed
Deamination of deoxycytidine n ...
Escherichia coli, Rickettsia prowazekii, Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
173
4902-4903
1991
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209517
Wang
5-Methyl-dCTP deaminase induce ...
Xanthomonas oryzae
J. Virol.
42
42-48
1982
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209518
Neuhard
dCTP Deaminase from Salmonella ...
Salmonella enterica subsp. enterica serovar Typhimurium
Methods Enzymol.
51
418-423
1978
-
-
-
-
-
-
3
-
-
3
1
-
1
2
-
-
1
-
-
-
1
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
3
1
-
1
-
-
1
-
-
1
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
209519
Beck
Deoxycytidine triphosphate dea ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
250
609-616
1975
-
-
-
-
-
-
7
-
-
4
1
-
1
2
-
-
1
-
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
4
1
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
209520
Price
Bacteriophage PBS2-induced deo ...
Bacillus subtilis
J. Virol.
14
1314-1317
1974
-
-
-
-
-
-
7
1
-
1
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
1
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
209521
Tomita
A novel enzyme, dCTP deaminase ...
Bacillus subtilis
Biochim. Biophys. Acta
179
18-27
1969
-
-
-
-
-
-
3
1
-
1
-
-
-
2
-
-
1
-
-
-
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-