BRENDA - Reference to 3.5.4.13; Id = 686667

mutant E138A bound to dTTP and mutant H121A bound to dCTP, hanging drop vapour diffusion method, 3.7 mg/ml E138A or 5.1 mg/ml H121A in 20 mM magnesium chloride, 50 mM HEPES, pH 6.8, and 5 mM nucleotide, mixing of 0.002 ml of protein solution with an equal volume of reservoir solution containing 34% PEG 400, 0.2 mM MgCl2, and 0.1 m HEPES, pH 7.5, equilibration against 1 ml of mother liquor, room temperature, 1 week, X-ray diffraction structure determination and analysis at 2.6-2.7 A resolution

Escherichia coli

E138A

site-directed mutagenesis, inactive mutant, dTTP is bound to the active site of E138A, the region between Val120 and His125 is formed in a new conformation, the C-terminal fold is disordered

Escherichia coli

H121A

site-directed mutagenesis, inactive mutant, dTTP is bound to the active site of E138A, the region between Val120 and His125 is formed in a new conformation, the C-terminal fold is disordered

Escherichia coli

V122G

site-directed mutagenesis, inactive mutant

Escherichia coli

dTTP

inhibits the enzyme in a nonallosterical way, cooperative kinetics are imposed by a dTTP-dependent lag of product formation observed in presteady-state kinetics, the lag may be derived from a slow equilibration between an inactive and an active conformation of dCTP deaminase represented by the dTTP complex and the dUTP/dCTP complex, respectively, steady-state kinetic analysis of dTTP inhibition, overview

Escherichia coli

additional information

-

additional information

enzyme kinetics and equilibrium binding

Escherichia coli

Mg2+

-

Escherichia coli

dCTP + H2O

Escherichia coli

-

dUTP + NH3

-

?

Escherichia coli

P28248

-

dCTP + H2O

-

686667

Escherichia coli

dUTP + NH3

-

?

37

-

assay at

Escherichia coli

6.8

-

assay at

Escherichia coli

mutant E138A bound to dTTP and mutant H121A bound to dCTP, hanging drop vapour diffusion method, 3.7 mg/ml E138A or 5.1 mg/ml H121A in 20 mM magnesium chloride, 50 mM HEPES, pH 6.8, and 5 mM nucleotide, mixing of 0.002 ml of protein solution with an equal volume of reservoir solution containing 34% PEG 400, 0.2 mM MgCl2, and 0.1 m HEPES, pH 7.5, equilibration against 1 ml of mother liquor, room temperature, 1 week, X-ray diffraction structure determination and analysis at 2.6-2.7 A resolution

Escherichia coli

E138A

site-directed mutagenesis, inactive mutant, dTTP is bound to the active site of E138A, the region between Val120 and His125 is formed in a new conformation, the C-terminal fold is disordered

Escherichia coli

H121A

site-directed mutagenesis, inactive mutant, dTTP is bound to the active site of E138A, the region between Val120 and His125 is formed in a new conformation, the C-terminal fold is disordered

Escherichia coli

V122G

site-directed mutagenesis, inactive mutant

Escherichia coli

dTTP

inhibits the enzyme in a nonallosterical way, cooperative kinetics are imposed by a dTTP-dependent lag of product formation observed in presteady-state kinetics, the lag may be derived from a slow equilibration between an inactive and an active conformation of dCTP deaminase represented by the dTTP complex and the dUTP/dCTP complex, respectively, steady-state kinetic analysis of dTTP inhibition, overview

Escherichia coli

additional information

-

additional information

enzyme kinetics and equilibrium binding

Escherichia coli

Mg2+

-

Escherichia coli

dCTP + H2O

Escherichia coli

-

dUTP + NH3

-

?

dCTP + H2O

-

686667

Escherichia coli

dUTP + NH3

-

?

37

-

assay at

Escherichia coli

6.8

-

assay at

Escherichia coli

733177

Oehlenschlaeger

Bacillus halodurans strain C12 ...

Bacillus halodurans, Bacillus halodurans C-125

Appl. Environ. Microbiol.

81

3395-3404

2015

1

-

1

-

1

-

2

-

12

-

1

-

2

-

2

-

1

-

1

-

1

-

1

-

2

-

1

-

2

-

684700

Thymark

Mutational analysis of the nuc ...

Escherichia coli

Arch. Biochem. Biophys.

470

20-26

2008

-

1

4

-

1

-

1

-

1

-

3

-

2

-

1

-

2

1

-

1

4

-

1

-

1

-

1

-

2

-

1

-

2

1

-

688399

Helt

Mechanism of dTTP inhibition o ...

Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv

J. Mol. Biol.

376

554-569

2008

-

1

-

1

-

6

-

1

-

2

-

3

-

1

-

1

-

1

-

2

-

686667

Johansson

Regulation of dCTP deaminase f ...

Escherichia coli

FEBS J.

274

4188-4198

2007

-

1

3

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

1

3

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

688732

Zhang

Chloroviruses encode a bifunct ...

Paramecium bursaria Chlorella virus 1

J. Virol.

81

7662-7671

2007

1

-

1

-

3

2

-

5

1

2

-

4

-

1

-

1

4

1

-

2

1

-

1

-

1

-

3

-

2

-

5

1

2

-

1

-

1

4

1

-

2

1

-

669375

Johansson

Structures of dCTP deaminase f ...

Escherichia coli

J. Biol. Chem.

280

3051-3059

2005

-

1

3

-

3

-

1

-

1

3

-

648212

Huffman

Structural basis for recogniti ...

Methanocaldococcus jannaschii

J. Mol. Biol.

331

885-896

2003

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

652395

Bjornberg

A bifunctional dCTP deaminase- ...

Methanocaldococcus jannaschii

J. Biol. Chem.

278

20667-20672

2003

-

1

-

2

1

-

1

2

1

-

4

-

1

-

4

1

-

2

-

1

-

2

1

-

1

2

1

-

1

-

4

1

-

209525

Estevenon

An Escherichia coli strain def ...

Escherichia coli

Mol. Gen. Genet.

246

514-518

1995

-

1

-

2

-

1

-

209524

Weiss

De novo synthesis of thymidyla ...

Escherichia coli

J. Bacteriol.

176

2194-2199

1994

-

2

-

1

-

1

-

209522

Wang

Dcd (dCTP deaminase) gene of E ...

Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium

J. Bacteriol.

174

5647-5653

1992

-

2

-

1

-

5

-

2

-

2

-

1

-

2

-

209523

Speed

Deamination of deoxycytidine n ...

Escherichia coli, Rickettsia prowazekii, Salmonella enterica subsp. enterica serovar Typhimurium

J. Bacteriol.

173

4902-4903

1991

-

5

-

3

-

3

-

209517

Wang

5-Methyl-dCTP deaminase induce ...

Xanthomonas oryzae

J. Virol.

42

42-48

1982

-

3

-

1

-

4

-

1

-

1

-

1

-

4

-

209518

Neuhard

dCTP Deaminase from Salmonella ...

Salmonella enterica subsp. enterica serovar Typhimurium

Methods Enzymol.

51

418-423

1978

-

3

-

3

1

-

1

2

-

1

-

1

-

1

-

1

-

1

-

3

-

3

1

-

1

-

1

-

1

-

1

-

1

-

1

-

209519

Beck

Deoxycytidine triphosphate dea ...

Salmonella enterica subsp. enterica serovar Typhimurium

J. Biol. Chem.

250

609-616

1975

-

7

-

4

1

-

1

2

-

1

-

1

-

1

-

1

-

7

-

4

1

-

1

-

1

-

1

-

1

-

1

-

209520

Price

Bacteriophage PBS2-induced deo ...

Bacillus subtilis

J. Virol.

14

1314-1317

1974

-

7

1

-

1

-

3

-

1

-

1

-

1

-

7

-

1

-

1

-

1

-

1

-

1

-

209521

Tomita

A novel enzyme, dCTP deaminase ...

Bacillus subtilis

Biochim. Biophys. Acta

179

18-27

1969

-

3

1

-

1

-

2

-

1

-

1

-

1

-

1

-

3

-

1

-

1

-

1

-

1

-

1

-

1

-

Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme

Data extracted from this reference: