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Literature summary for 3.5.4.12 extracted from
- The first crystal structure of a dTTP-bound deoxycytidylate deaminase validates and details the allosteric-inhibitor binding site (2015), J. Biol. Chem., 290, 682-690.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Cyanophage S-TIM5 |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| bound to dTTP, hanging drop vapor diffusion method, using 0.2 M potassium formate, 20% (w/v) PEG 3350 | Cyanophage S-TIM5 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N43G | the mutation results in a dramatic loss of activity with less than 20% activity compared to the wild type | Cyanophage S-TIM5 |
| T61A | the mutant shows about 65% activity compared to the wild type enzyme | Cyanophage S-TIM5 |
| T61D | the mutant shows about 7% activity compared to the wild type enzyme | Cyanophage S-TIM5 |
| T61S | the mutant shows about 145% activity compared to the wild type enzyme | Cyanophage S-TIM5 |
| T61V | the mutant shows about 50% activity compared to the wild type enzyme | Cyanophage S-TIM5 |
| W42A | inactive | Cyanophage S-TIM5 |
| W42F | the mutant shows about 118% activity compared to the wild type enzyme | Cyanophage S-TIM5 |
| W42Y | the mutant shows about wild type activity | Cyanophage S-TIM5 |
| Y116E | inactive | Cyanophage S-TIM5 |
| Y116F | the mutant shows about 140% activity compared to the wild type enzyme | Cyanophage S-TIM5 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dTTP | - |
Cyanophage S-TIM5 |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Cyanophage S-TIM5 | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dCMP + H2O | Cyanophage S-TIM5 | - |
dUMP + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyanophage S-TIM5 | H6WFU3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisPur cobalt resin column chromatography and Superdex 200 gel filtration | Cyanophage S-TIM5 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dCMP + H2O | - |
Cyanophage S-TIM5 | dUMP + NH3 | - |
? | |
| additional information | no deamination activity with dCTP or CMP | Cyanophage S-TIM5 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DCD | - |
Cyanophage S-TIM5 |
| deoxycytidylate deaminase | - |
Cyanophage S-TIM5 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Cyanophage S-TIM5 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | more than 80% activity between pH 6.0 and 8.0 | Cyanophage S-TIM5 |
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Release 2023.1 (January 2023)
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