Literature summary for 3.5.4.12 extracted from

Nucci, R.; Raia, C.A.; Vaccaro, C.; Rossi, M.; Whitehead, E.P.
Allosteric modifier and substrate binding of donkey deoxycytidylate aminohydrolase (EC 3.5.4.12) (1991), Arch. Biochem. Biophys., 289, 19-25.

Search for more literature for 3.5.4.12

Activating Compound

Activating Compound	Comment	Organism	Structure
dCTP	positive allosteric effector	Equus asinus
additional information	mechanism of activation	Equus asinus

General Stability

General Stability	Organism
glycerol stabilizes	Equus asinus

Inhibitors

Inhibitors	Comment	Organism	Structure
dAMP	kinetics of dAMP inhibition	Equus asinus
dTTP	allosteric inhibitor; cooperative inhibition, reversed by dCTP, mechanism of inhibition	Equus asinus
additional information	mechanism of inhibition	Equus asinus
UTP-adipic hydrazide	cooperative inhibition, reversed by dCTP	Equus asinus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	cooperative kinetics, kinetic affinities and effects, mechanism	Equus asinus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
18842	-	6 * 18842, amino acid analysis	Equus asinus
113100	-	amino acid analysis	Equus asinus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dCMP + H2O	Equus asinus	-	dUMP + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Equus asinus	-	donkey	-

Purification (Commentary)

Purification (Comment)	Organism
-	Equus asinus

Reaction

Reaction	Comment	Organism	Reaction ID
dCMP + H2O = dUMP + NH3	mechanism	Equus asinus	a
dCMP + H2O = dUMP + NH3	regulation, allosteric end-product regulation	Equus asinus	a
dCMP + H2O = dUMP + NH3	feedback regulation	Equus asinus	a
dCMP + H2O = dUMP + NH3	highly regulated allosteric enzyme	Equus asinus	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
spleen	-	Equus asinus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
730	-	-	Equus asinus

Storage Stability

Storage Stability	Organism
-20°C, 0.1 M Na-phosphate, pH 7.5, 50% glycerol, 12 months, no activity lost	Equus asinus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-methyl-dCMP + H2O	-	Equus asinus	5-methyl-dUMP + NH3	-	?
dCMP + H2O	-	Equus asinus	dUMP + NH3	-	?

Subunits

Subunits	Comment	Organism
hexamer	6 identical subunits	Equus asinus
hexamer	6 * 18842, amino acid analysis	Equus asinus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
38	-	assay at	Equus asinus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1400	-	dCMP	-	Equus asinus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)