BRENDA - Enzyme Database
show all sequences of 3.5.4.12

Deoxycytidylate deaminase. Purification and some properties of the enzyme isolated from human spleen

Ellims, P.H.; Kao, A.Y.; Chabner, B.A.; J. Biol. Chem. 256, 6335-6340 (1981)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
1-beta-D-arabinofuranosylcytosine 5'-triphosphate
low activation, allosteric activator
Homo sapiens
CDP
low activation, allosteric activator
Homo sapiens
CTP
low activation, allosteric activator
Homo sapiens
dAMP
0.025 mM: low activation
Homo sapiens
dCDP
low activation, allosteric activator
Homo sapiens
dCTP
activation by dCTP has absolute requirement for the presence of a divalent cation, Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP; allosteric activator; less than 0.001 mM: very high activation
Homo sapiens
TTP
activating enzyme up to 1.7fold at concentration of 0.00025 mM, but inhibiting at higher concentration, 0.015 mM: 85% inhibition
Homo sapiens
General Stability
General Stability
Organism
dCTP and MgCl2 stabilize
Homo sapiens
dithiothreitol, 2 mM, stabilizes
Homo sapiens
glycerol, 15%, stabilizes
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP
slight inhibition
Homo sapiens
AMP
slight inhibition, competitive inhibitor, mechanism of inhibition
Homo sapiens
Co2+
1 mM: 75% inhibition
Homo sapiens
Cu2+
1 mM: 98% inhibition
Homo sapiens
dGDP
slight inhibition
Homo sapiens
dGMP
effective competitive inhibitor, mechanism of inhibition
Homo sapiens
dUMP
poor competitive inhibitor, mechanism of inhibition
Homo sapiens
dUTP
slight inhibition
Homo sapiens
EDTA
no inhibition by EDTA
Homo sapiens
GMP
slight inhibition
Homo sapiens
additional information
no inhibition by EDTA
Homo sapiens
TDP
slight inhibition, competitive inhibitor, mechanism of inhibition
Homo sapiens
TMP
poor competitive inhibitor, mechanism of inhibition
Homo sapiens
TTP
activating enzyme up to 1.7fold at concentration of 0.00025 mM, but inhibiting at higher concentration, 0.015 mM: 85% inhibition; inhibition by TTP has absolute requirement for the presence of a divalent cation, Mn2+ and Ca2+ are more effective than Mg2+ in the inhibition of enzyme activity by TTP; TTP inhibition partially reversed by dCTP
Homo sapiens
UTP
slight inhibition
Homo sapiens
Zn2+
1 mM: 94% inhibition
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic behavior and data
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP
Homo sapiens
Mg2+
activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP
Homo sapiens
Mn2+
activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP
Homo sapiens
additional information
metal ions not required
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
2 * 53000, SDS-PAGE
Homo sapiens
109500
-
gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
dCMP + H2O
Homo sapiens
important role in regulation of intracellular TTP levels
dUMP + NH3
-
-
ir
dCMP + H2O
Homo sapiens
enzyme is major source of mammalian cell dUMP pool and regulates the synthesis of TMP
dUMP + NH3
-
-
ir
Organism
Organism
UniProt
Commentary
Textmining
Homo sapiens
-
human
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Homo sapiens
Reaction
Reaction
Commentary
Organism
Reaction ID
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
spleen
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
10
-
-
Homo sapiens
Storage Stability
Storage Stability
Organism
-50°C, 0.05 mM dCTP, 1 mM MgCl2 or 2 mM dithiothreitol, 18 h, 40% activity lost
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ara-CMP + H2O
arabinosyl-CMP
288999
Homo sapiens
ara-UMP + NH3
-
-
-
?
ara-CMP + H2O
1-beta-D-arabinofuranosyl-CMP
288999
Homo sapiens
ara-UMP + NH3
-
-
-
?
ara-CMP + H2O
effective substrate in presence of dCTP
288999
Homo sapiens
ara-UMP + NH3
-
-
-
?
CMP + H2O
poor substrate
288999
Homo sapiens
UMP + NH3
-
-
-
?
dCMP + H2O
high degree of specificity of dCMP
288999
Homo sapiens
dUMP + NH3
-
288999
Homo sapiens
ir
dCMP + H2O
important role in regulation of intracellular TTP levels
288999
Homo sapiens
dUMP + NH3
-
-
-
ir
dCMP + H2O
enzyme is major source of mammalian cell dUMP pool and regulates the synthesis of TMP
288999
Homo sapiens
dUMP + NH3
-
-
-
ir
additional information
substrate specificity
288999
Homo sapiens
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 53000, SDS-PAGE
Homo sapiens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 5.10
Homo sapiens
6.5
7.3
broad pH optimum
Homo sapiens
7.2
-
assay at
Homo sapiens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
1-beta-D-arabinofuranosylcytosine 5'-triphosphate
low activation, allosteric activator
Homo sapiens
CDP
low activation, allosteric activator
Homo sapiens
CTP
low activation, allosteric activator
Homo sapiens
dAMP
0.025 mM: low activation
Homo sapiens
dCDP
low activation, allosteric activator
Homo sapiens
dCTP
activation by dCTP has absolute requirement for the presence of a divalent cation, Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP; allosteric activator; less than 0.001 mM: very high activation
Homo sapiens
TTP
activating enzyme up to 1.7fold at concentration of 0.00025 mM, but inhibiting at higher concentration, 0.015 mM: 85% inhibition
Homo sapiens
General Stability (protein specific)
General Stability
Organism
dCTP and MgCl2 stabilize
Homo sapiens
dithiothreitol, 2 mM, stabilizes
Homo sapiens
glycerol, 15%, stabilizes
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP
slight inhibition
Homo sapiens
AMP
slight inhibition, competitive inhibitor, mechanism of inhibition
Homo sapiens
Co2+
1 mM: 75% inhibition
Homo sapiens
Cu2+
1 mM: 98% inhibition
Homo sapiens
dGDP
slight inhibition
Homo sapiens
dGMP
effective competitive inhibitor, mechanism of inhibition
Homo sapiens
dUMP
poor competitive inhibitor, mechanism of inhibition
Homo sapiens
dUTP
slight inhibition
Homo sapiens
EDTA
no inhibition by EDTA
Homo sapiens
GMP
slight inhibition
Homo sapiens
additional information
no inhibition by EDTA
Homo sapiens
TDP
slight inhibition, competitive inhibitor, mechanism of inhibition
Homo sapiens
TMP
poor competitive inhibitor, mechanism of inhibition
Homo sapiens
TTP
activating enzyme up to 1.7fold at concentration of 0.00025 mM, but inhibiting at higher concentration, 0.015 mM: 85% inhibition; inhibition by TTP has absolute requirement for the presence of a divalent cation, Mn2+ and Ca2+ are more effective than Mg2+ in the inhibition of enzyme activity by TTP; TTP inhibition partially reversed by dCTP
Homo sapiens
UTP
slight inhibition
Homo sapiens
Zn2+
1 mM: 94% inhibition
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic behavior and data
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP
Homo sapiens
Mg2+
activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP
Homo sapiens
Mn2+
activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP
Homo sapiens
additional information
metal ions not required
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
2 * 53000, SDS-PAGE
Homo sapiens
109500
-
gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
dCMP + H2O
Homo sapiens
important role in regulation of intracellular TTP levels
dUMP + NH3
-
-
ir
dCMP + H2O
Homo sapiens
enzyme is major source of mammalian cell dUMP pool and regulates the synthesis of TMP
dUMP + NH3
-
-
ir
Purification (Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
spleen
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
10
-
-
Homo sapiens
Storage Stability (protein specific)
Storage Stability
Organism
-50°C, 0.05 mM dCTP, 1 mM MgCl2 or 2 mM dithiothreitol, 18 h, 40% activity lost
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ara-CMP + H2O
arabinosyl-CMP
288999
Homo sapiens
ara-UMP + NH3
-
-
-
?
ara-CMP + H2O
1-beta-D-arabinofuranosyl-CMP
288999
Homo sapiens
ara-UMP + NH3
-
-
-
?
ara-CMP + H2O
effective substrate in presence of dCTP
288999
Homo sapiens
ara-UMP + NH3
-
-
-
?
CMP + H2O
poor substrate
288999
Homo sapiens
UMP + NH3
-
-
-
?
dCMP + H2O
high degree of specificity of dCMP
288999
Homo sapiens
dUMP + NH3
-
288999
Homo sapiens
ir
dCMP + H2O
important role in regulation of intracellular TTP levels
288999
Homo sapiens
dUMP + NH3
-
-
-
ir
dCMP + H2O
enzyme is major source of mammalian cell dUMP pool and regulates the synthesis of TMP
288999
Homo sapiens
dUMP + NH3
-
-
-
ir
additional information
substrate specificity
288999
Homo sapiens
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 53000, SDS-PAGE
Homo sapiens
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 5.10
Homo sapiens
6.5
7.3
broad pH optimum
Homo sapiens
7.2
-
assay at
Homo sapiens
Other publictions for EC 3.5.4.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734322
Marx
The first crystal structure of ...
Cyanophage S-TIM5
J. Biol. Chem.
290
682-690
2015
-
-
1
1
10
-
1
-
-
1
-
1
-
2
-
-
1
-
-
-
-
-
2
-
2
-
-
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1
1
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1
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1
10
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1
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
734401
Xu
STRIPE2 encodes a putative dCM ...
Oryza sativa
J. Genet. Genomics
41
539-548
2014
-
-
1
-
-
-
-
-
2
-
-
1
-
2
-
-
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1
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1
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3
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1
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2
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1
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712083
Jansen
Deoxyuridine analog nucleotide ...
Homo sapiens
Fundam. Clin. Pharmacol.
25
172-185
2011
1
-
-
-
-
-
8
-
-
-
-
1
-
1
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-
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7
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2
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1
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8
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1
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7
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
711628
Li
DNA mismatch repair (MMR)-depe ...
Homo sapiens
Br. J. Pharmacol.
158
679-692
2009
-
-
-
-
-
-
1
-
-
-
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1
-
1
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1
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1
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-
-
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-
688405
Hou
Crystal structures of Streptoc ...
Streptococcus mutans
J. Mol. Biol.
377
220-231
2008
1
-
1
1
-
-
1
-
-
5
-
1
-
2
-
-
1
-
-
-
-
-
2
2
2
1
-
-
-
1
-
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1
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1
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1
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1
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5
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1
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1
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2
2
1
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1
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-
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-
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-
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-
686248
Liskay
Involvement of deoxycytidylate ...
Saccharomyces cerevisiae
Curr. Biol.
17
R755-R757
2007
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2
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1
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2
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1
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-
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-
688732
Zhang
Chloroviruses encode a bifunct ...
Paramecium bursaria Chlorella virus 1
J. Virol.
81
7662-7671
2007
1
-
1
-
-
-
3
2
-
5
1
2
-
4
-
-
1
-
-
-
-
1
4
1
1
1
1
-
2
2
1
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1
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1
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3
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2
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5
1
2
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1
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1
4
1
1
1
-
2
2
1
-
-
-
-
-
-
-
-
667587
Almog
Three-dimensional structure of ...
Escherichia virus T4
Biochemistry
43
13715-13723
2004
1
1
1
1
1
-
2
-
-
1
-
1
-
1
-
-
1
1
-
-
-
-
2
1
2
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1
1
1
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1
1
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2
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1
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1
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1
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-
2
1
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-
-
-
-
-
-
-
-
-
-
669680
Gribaudo
Human cytomegalovirus requires ...
Homo sapiens
J. Gen. Virol.
84
1437-1441
2003
1
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-
-
-
-
-
-
-
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2
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3
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1
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3
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2
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1
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2
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1
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3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670328
Liou
Assessment of the effect of ph ...
Homo sapiens
Mol. Pharmacol.
63
105-110
2003
-
-
-
-
-
-
10
1
-
1
-
1
-
3
-
-
1
-
-
1
-
-
4
-
2
-
-
-
-
1
-
-
-
6
-
-
-
-
-
-
-
-
-
-
10
6
1
-
1
-
1
-
-
-
1
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1
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4
-
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-
-
1
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-
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-
-
-
-
-
667541
Cha
Modifications of deoxycytidine ...
Rattus norvegicus
Biochem. Pharmacol.
63
717-723
2002
1
-
-
-
-
-
1
4
-
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1
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1
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3
1
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2
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2
1
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1
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1
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1
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4
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1
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3
1
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2
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1
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1
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-
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-
-
-
-
-
-
668584
Jost
5-Methyldeoxycytidine monophos ...
Homo sapiens
FEBS Lett.
519
128-134
2002
1
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-
-
-
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1
1
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1
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3
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2
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1
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2
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5
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2
1
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1
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1
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1
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1
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5
-
1
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1
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-
-
289015
Keefe
A T4-phage deoxycytidylate dea ...
Enterobacteria phage T2, Enterobacteria phage T6, Escherichia virus T4
J. Biol. Chem.
275
12598-12602
2000
7
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1
-
3
4
4
5
-
4
9
2
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4
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1
3
1
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4
2
4
5
2
1
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5
2
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7
-
1
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3
4
-
4
-
5
-
4
9
2
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1
1
-
4
2
4
5
1
-
-
5
2
-
-
-
-
-
-
-
-
-
289014
Nucci
Interaction of the high-affini ...
Equus asinus
Arch. Biochem. Biophys.
310
49-53
1994
2
1
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-
-
-
3
1
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1
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1
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1
1
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1
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1
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1
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1
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1
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1
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1
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1
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-
-
289017
Moore
Identification of a site neces ...
Escherichia virus T4, Homo sapiens
Biochemistry
33
2104-2112
1994
3
-
2
-
1
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6
-
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1
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2
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2
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1
2
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4
2
3
2
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3
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2
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1
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6
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1
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2
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1
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4
2
2
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-
289018
Maley
Properties of an affinity-colu ...
Homo sapiens
Biochim. Biophys. Acta
1162
161-170
1993
2
-
1
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-
5
11
-
-
7
2
1
-
3
-
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1
1
-
1
1
1
10
1
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1
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1
2
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2
-
1
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-
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5
-
11
-
-
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7
2
1
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1
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1
1
1
10
1
1
-
-
1
2
-
-
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-
-
-
-
289016
Xu
Modulation of deoxycytidylate ...
Homo sapiens
Biochem. Pharmacol.
44
1819-1827
1992
1
1
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7
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2
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2
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1
1
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3
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4
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1
1
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7
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2
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1
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3
-
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4
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
289012
Nucci
Allosteric modifier and substr ...
Equus asinus
Arch. Biochem. Biophys.
289
19-25
1991
2
-
-
-
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1
4
1
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2
1
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3
-
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1
1
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3
1
1
2
1
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1
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1
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2
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-
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1
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4
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1
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2
1
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1
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3
1
1
2
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
289013
Whitehead
Hill coefficient ratios give b ...
Equus asinus
Arch. Biochem. Biophys.
289
12-18
1991
2
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2
1
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1
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1
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1
1
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1
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1
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1
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1
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2
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2
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1
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1
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1
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1
-
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1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
289019
Riva
-
Correlation between deoxycytid ...
Homo sapiens
J. Cell. Pharmacol.
1
79-85
1990
-
-
-
-
-
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1
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-
-
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1
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1
-
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2
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1
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1
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1
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2
-
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1
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-
-
-
-
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-
-
-
289020
Maley
A tale of two enzymes, deoxycy ...
Bacillus subtilis, Enterobacteria phage T2, Enterobacteria phage T6, Equus asinus, Escherichia virus T4, Gallus gallus, Herpes simplex virus, Homo sapiens, Macaca mulatta polyomavirus 1, no activity in Escherichia coli, no activity in Salmonella typhimurium, Polyomavirus sp., Rattus norvegicus, Saccharomyces cerevisiae, Sea urchin
Prog. Nucleic Acid Res. Mol. Biol.
39
49-80
1990
10
3
1
1
-
6
8
8
-
-
1
28
-
15
-
-
2
8
-
20
1
-
70
7
-
2
-
-
-
-
-
-
-
-
-
-
10
3
1
-
1
-
6
-
8
-
8
-
-
1
28
-
-
-
2
-
20
1
-
70
7
2
-
-
-
-
-
-
-
-
-
-
-
-
-
288997
Maley
Probing the infra-structure of ...
Enterobacteria phage T2, Gallus gallus
Adv. Enzyme Regul.
22
413-430
1984
3
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1
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2
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8
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5
-
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1
2
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1
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10
2
3
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3
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1
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2
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8
-
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1
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1
-
-
10
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289000
McIntosh
Isolation of a Saccharomyces c ...
no activity in Escherichia coli, no activity in Salmonella typhimurium, Saccharomyces cerevisiae
J. Bacteriol.
158
644-649
1984
1
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2
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1
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1
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5
-
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1
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9
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2
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1
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-
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-
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2
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1
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1
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-
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1
-
-
-
1
9
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
209853
Neale
Enzymes of pyrimidine deoxyrib ...
Mycoplasma mycoides
J. Bacteriol.
156
1001-1005
1983
1
-
-
-
-
1
1
2
-
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1
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3
-
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1
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2
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1
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1
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1
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1
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1
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2
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1
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-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
289002
Mastrantonio
Analysis of competition for su ...
Equus asinus
Eur. J. Biochem.
137
421-427
1983
2
-
-
-
-
-
3
3
-
-
1
1
-
2
-
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1
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2
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1
1
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2
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3
-
3
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1
1
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-
-
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2
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289003
Raia
Reversal of the effect of the ...
Equus asinus
J. Mol. Biol.
157
557-570
1982
5
-
-
-
-
1
8
2
-
-
1
1
-
1
-
-
1
1
-
1
3
-
15
1
-
1
-
-
-
1
-
-
-
-
-
-
5
-
-
-
-
-
1
-
8
-
2
-
-
1
1
-
-
-
1
-
1
3
-
15
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
288999
Ellims
Deoxycytidylate deaminase. Pur ...
Homo sapiens
J. Biol. Chem.
256
6335-6340
1981
7
-
-
-
-
3
16
1
-
4
2
2
-
2
-
-
1
1
-
2
1
1
8
1
-
1
-
-
-
3
-
-
-
-
-
-
7
-
-
-
-
-
3
-
16
-
1
-
4
2
2
-
-
-
1
-
2
1
1
8
1
1
-
-
-
3
-
-
-
-
-
-
-
-
-
288998
Nucci
Freezing of dCMP aminohydrolas ...
Equus asinus
J. Mol. Biol.
124
133-145
1978
2
-
-
-
-
-
3
1
-
1
2
1
-
1
-
-
1
1
-
1
2
-
6
1
-
1
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
3
-
1
-
1
2
1
-
-
-
1
-
1
2
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
289004
Maley
Deoxycytidylate deaminase from ...
Enterobacteria phage T2, no activity in Escherichia coli
Methods Enzymol.
51
412-418
1978
2
-
-
-
-
3
1
4
-
4
1
2
-
3
-
-
1
1
-
-
1
1
12
1
-
-
-
1
-
3
-
-
-
-
-
-
2
-
-
-
-
-
3
-
1
-
4
-
4
1
2
-
-
-
1
-
-
1
1
12
1
-
-
1
-
3
-
-
-
-
-
-
-
-
-
289005
Mollgaard
Deoxycytidylate deaminase from ...
Bacillusphage phiE, Bacillus subtilis, Bacillus subtilis ED40, bacteriophage SP8, no activity in Escherichia coli, no activity in Salmonella typhimurium
J. Biol. Chem.
253
3536-3542
1978
9
-
-
-
-
4
2
2
-
10
1
2
-
7
-
-
1
1
-
-
1
3
12
-
-
1
-
2
-
1
1
-
-
-
-
-
9
-
-
-
-
-
4
-
2
-
2
-
10
1
2
-
-
-
1
-
-
1
3
12
-
1
-
2
-
1
1
-
-
-
-
-
-
-
-
289006
Dosseva.I.M.; Tomov
-
Stabilizing effect of ethylene ...
Mus musculus
Dokl. Bolg. Akad. Nauk
28
241-244
1975
-
-
-
-
-
5
-
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
289007
Rolton
Deoxycytidylate deaminase. Evi ...
Cricetinae, Herpes simplex virus
Biochem. J.
143
403-409
1974
6
-
-
-
-
3
6
4
-
2
-
2
-
2
-
-
2
-
-
2
-
-
4
-
-
2
-
4
-
2
-
-
-
-
-
-
6
-
-
-
-
-
3
-
6
-
4
-
2
-
2
-
-
-
2
-
2
-
-
4
-
2
-
4
-
2
-
-
-
-
-
-
-
-
-
289008
Rolton
Deoxycytidylate deaminase. Pro ...
Cricetinae
Biochem. J.
141
211-217
1974
1
-
-
-
-
3
2
1
-
3
2
1
-
1
-
-
1
1
-
2
1
2
4
-
-
1
-
2
-
1
1
-
1
-
-
-
1
-
-
1
-
-
3
-
2
-
1
-
3
2
1
-
-
-
1
-
2
1
2
4
-
1
-
2
-
1
1
-
-
-
-
-
-
-
-
289009
Maley
T2r+ bacteriophage-induced enz ...
Enterobacteria phage T2, Escherichia virus T4, Gallus gallus
J. Biol. Chem.
247
931-939
1972
5
-
-
-
-
2
3
4
-
-
1
3
-
3
-
-
2
3
-
1
1
2
11
3
-
2
-
-
-
4
-
-
-
-
-
-
5
-
-
-
-
-
2
-
3
-
4
-
-
1
3
-
-
-
2
-
1
1
2
11
3
2
-
-
-
4
-
-
-
-
-
-
-
-
-
289010
Maley
T2r+ bacteriophage-induced enz ...
Enterobacteria phage T2, Gallus gallus
J. Biol. Chem.
247
940-945
1972
3
-
-
-
-
2
3
-
-
-
2
2
-
2
-
-
-
2
1
1
-
-
4
2
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
3
-
-
-
-
2
2
-
-
-
-
1
1
-
-
4
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289011
Sergott
On the regulation of a bacteri ...
Lactobacillus acidophilus, Staphylococcus aureus
J. Biol. Chem.
246
7755-7758
1971
4
-
-
-
-
1
6
-
-
-
-
2
-
2
-
-
2
2
-
-
1
1
4
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
6
-
-
-
-
-
2
-
-
-
2
-
-
1
1
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-