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Literature summary for 3.5.4.10 extracted from
- 5-Aminoimidazole-4-carboxamide ribotide transformylase-IMP cyclohydrolase from human CCRF-CEM leukemia cells: purification, pH dependence and inhibitors (1994), Biochemistry, 33, 14237-14245.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2'-deoxy-AMP | decreased IMP cyclohydrolase activity by 10-50% | Homo sapiens |  |
| 2-fluoroadenine arabinoside 5'-monophosphate | IC50-value in cell culture 0.0002 mM; strong competitive inhibitor, derivative of anti-cancer drug | Homo sapiens | |
| 2-mercaptoinosine 5'-monophosphate | most potent competitive inhibitor, precursor of GMP in the purine pathway | Homo sapiens | |
| 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside | decreased IMP cyclohydrolase activity by 10-50% | Homo sapiens | |
| 6-mercaptopurine riboside 5'-monophosphate | IC50-value in cell culture 0.0075 mM; strong competitive inhibitor, derivative of anti-cancer drug | Homo sapiens | |
| adenosine N1-oxide 5'-monophosphate | IC50-value in cell culture 0.0011 microM; strong competitive inhibitor | Homo sapiens | |
| AMP | decreased IMP cyclohydrolase activity by 10-50% | Homo sapiens | |
| GMP | decreased IMP cyclohydrolase activity by 10-50% | Homo sapiens | |
| IMP | decreased IMP cyclohydrolase activity by 10-50% | Homo sapiens | |
| N-succino-AMP | decreased IMP cyclohydrolase activity by 10-50% | Homo sapiens | |
| N2-acetyl-2'deoxyguanosine 5'-monophosphate | decreased IMP cyclohydrolase activity by 10-50% | Homo sapiens | |
| N6-(carboxymethyl)adenosine 5'-monophosphate | strong competitive inhibitor | Homo sapiens | |
| xanthosine 5'-monophosphate | strong competitive inhibitor | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62100 | - |
2 * 62100, protein mobility assay | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | Homo sapiens | de novo purine synthesis step 10 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 780fold to apparent homogeneity | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| CEM cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | de novo purine synthesis step 10 | Homo sapiens | ? | - |
? | |
| 5-formamidoimidazole-4-carboxamide | - |
Homo sapiens | hypoxanthine + H2O | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 62100, protein mobility assay | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0000011 | - |
IC50-value in cell culture 0.0011 microM | Homo sapiens | adenosine N1-oxide 5'-monophosphate | |
| 0.0002 | - |
IC50-value in cell culture 0.0002 mM | Homo sapiens | 2-fluoroadenine arabinoside 5'-monophosphate | |
| 0.0075 | - |
IC50-value in cell culture 0.0075 mM | Homo sapiens | 6-mercaptopurine riboside 5'-monophosphate | |
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