Application | Comment | Organism |
---|---|---|
pharmacology | negative selection system for actinobacteria based on cytosine deaminase | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
pMP201 vector and derivatives with aminoglycoside resistance gene, synthetic version of the cytosine deaminase gene (CodA), optimized for expression in Streptomyces species, generation of Streptomyces lividans TK24 protoplasts, transformation of Streptomyces lividans | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D314A | PCR-directed mutagenesis, activity against 5-fluorocytosine 2-fold increased, 17-fold lower sensitivity against native cytosine | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorocytosine + H2O | Escherichia coli | used as negative selection marker system, increased 5-fluorocytosine sensitivity in Streptomyces lividans | 5-fluorouracil + NH3 | - |
? | |
cytosine + H2O | Escherichia coli | - |
uracil + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P25524 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
cytosine deaminase of Escherichia coli used as a negative selection marker in Streptomyces, can be applied for any actinobacterial species naturally resistant to 5-fluorocytosine while sensitive to 5-fluorouracil, valuable addition to the actinobacterial genetic toolbox | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorocytosine + H2O | used as negative selection marker system, increased 5-fluorocytosine sensitivity in Streptomyces lividans | Escherichia coli | 5-fluorouracil + NH3 | - |
? | |
cytosine + H2O | - |
Escherichia coli | uracil + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CodA protein | - |
Escherichia coli |