Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.4.1 extracted from

  • Yao, L.; Sklenak, S.; Yan, H.; Cukier, R.I.
    A molecular dynamics exploration of the catalytic mechanism of yeast cytosine deaminase (2005), J. Phys. Chem. B, 109, 7500-7510.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular dynamics simulation of free enzyme and in complex with cytosine, uracil and reaction intermediates Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ ligands are two cysteines, a histidine, and one water molecule Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Reaction

Reaction Comment Organism Reaction ID
cytosine + H2O = uracil + NH3 E64 side-chain carboxyl group acts as proton shuttle between the Zn-bound water molecule and cytosine. In course of reaction, cytosine reorients to favor nucleophilic attack by a Zn-bound hydroxide. F114 may be important for reactant binding and product release Saccharomyces cerevisiae