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Literature summary for 3.5.4.1 extracted from
- Purification and properties of intracellular cytosine deaminase from Chromobacterium violaceum YK 391 (2004), J. Microbiol. Biotechnol., 14, 1182-1189.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dipyridyl | - |
Chromobacterium violaceum |  |
| 2-thiocytosine | weak | Chromobacterium violaceum | |
| 2-Thiouracil | strong | Chromobacterium violaceum | |
| 5-Azacytosine | weak | Chromobacterium violaceum | |
| chloramine-T | - |
Chromobacterium violaceum | |
| Cu2+ | strong | Chromobacterium violaceum | |
| Fe3+ | strong | Chromobacterium violaceum | |
| Hg2+ | strong | Chromobacterium violaceum | |
| N-bromosuccinimide | - |
Chromobacterium violaceum | |
| o-phenanthroline | - |
Chromobacterium violaceum | |
| p-chloromercuribenzoate | - |
Chromobacterium violaceum | |
| Pb2+ | strong | Chromobacterium violaceum | |
| Zn2+ | strong | Chromobacterium violaceum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.38 | - |
cytosine | pH 7.5, 30°C | Chromobacterium violaceum | |
| 0.87 | - |
5-fluorocytosine | pH 7.5, 30°C | Chromobacterium violaceum | |
| 2.32 | - |
5-Methylcytosine | pH 7.5, 30°C | Chromobacterium violaceum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
2 * 63000, SDS-PAGE | Chromobacterium violaceum |
| 126000 | - |
- |
Chromobacterium violaceum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chromobacterium violaceum | - |
intracellular isoform | - |
| Chromobacterium violaceum YK 391 | - |
intracellular isoform | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Chromobacterium violaceum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-fluorocytosine + H2O | - |
Chromobacterium violaceum | 5-fluorouracil + NH3 | - |
? | |
| 5-fluorocytosine + H2O | - |
Chromobacterium violaceum YK 391 | 5-fluorouracil + NH3 | - |
? | |
| 5-methylcytosine + H2O | - |
Chromobacterium violaceum | thymine + NH3 | - |
? | |
| 5-methylcytosine + H2O | - |
Chromobacterium violaceum YK 391 | thymine + NH3 | - |
? | |
| 6-azacytosine + H2O | - |
Chromobacterium violaceum | 6-azauracil + NH3 | - |
? | |
| 6-azacytosine + H2O | - |
Chromobacterium violaceum YK 391 | 6-azauracil + NH3 | - |
? | |
| cytidine + H2O | - |
Chromobacterium violaceum | 1-beta-D-ribofuranosylpyrimidin-2(1H)-one + NH3 | - |
? | |
| cytosine + H2O | - |
Chromobacterium violaceum | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Chromobacterium violaceum YK 391 | uracil + NH3 | - |
? | |
| additional information | no substrate: 5-azacytosine | Chromobacterium violaceum | ? | - |
? | |
| additional information | no substrate: 5-azacytosine | Chromobacterium violaceum YK 391 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 63000, SDS-PAGE | Chromobacterium violaceum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Chromobacterium violaceum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
stable for a week | Chromobacterium violaceum |
| 60 | - |
5 min, 70% residual activity | Chromobacterium violaceum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Chromobacterium violaceum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Chromobacterium violaceum |
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