Literature summary for 3.5.4.1 extracted from

Porter, D.J.T.; Austin, E.A.
Cytosine deaminase the roles of divalent metal ions in catalysis (1993), J. Biol. Chem., 268, 24005-24011.

Application

Application	Comment	Organism
medicine	oral 5-fluorocytosine in combination with selective delivery of CDase to cancerous cells by selectively introducing the bacterial CDase gene into the genome	Escherichia coli

Cloned (Comment)	Organism
CDase gene cloned into pBS+ vector, gentically engineered Escherichia coli strain that overexpresses the codBA operon on a multicopy plasmid	Escherichia coli

Inhibitors	Comment	Organism
Cu2+	Fe2+CDase	Escherichia coli
H2O2	only Fe2+CDase, Mn2+CDase, Co2+CDase and Zn2+CDase are not inhibited	Escherichia coli
o-phenanthroline	-	Escherichia coli
Zn2+	Fe2+CDase	Escherichia coli

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.22	-	cytosine	-	Escherichia coli

Metals/Ions	Comment	Organism
Co2+	Mn2+ reconstitutes apoenzyme after removal of Fe2+	Escherichia coli
Fe2+	-	Escherichia coli
MnCl2	Mn2+ reconstitutes apoenzyme after removal of Fe2+	Escherichia coli
Zn2+	Mn2+ reconstitutes apoenzyme after removal of Fe2+	Escherichia coli

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48000	-	4 * 48000, SDS-PAGE	Escherichia coli

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cytosine + H2O	Escherichia coli	-	uracil + NH3	-	?

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Comment)	Organism
-	Escherichia coli

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2	-	+ 0.025 mM Cu(C2H3O2)	Escherichia coli
2.6	-	+ 0.025 mM HgCl2	Escherichia coli
2.6	-	+ 0.025 mM Pb(C2H3O2)	Escherichia coli
2.7	-	+ 0.025 mM CrCl2	Escherichia coli
2.9	-	apoCDase, + 0.025 SnCl2	Escherichia coli
3.1	-	+ 0.025 mM Cu(C2H3O2)	Escherichia coli
3.2	-	+ 0.025 mM CaCl2	Escherichia coli
4	-	+ 0.025 mM NiCl2, additional incubation with 0.025 mM Fe2+	Escherichia coli
5.6	-	+ 0.025 mM NiCl2	Escherichia coli
8.3	-	+ 0.025 mM Pb(C2H3O2), additional incubation of 0.025 mM Fe2+	Escherichia coli
11	-	+ 0.025 mM HgCl2, additional incubation with 0.025 mM Fe2+	Escherichia coli
12	-	+ 0.025 mM Zn(C2H3O2), additional incubation with 0.025 mM Fe2+	Escherichia coli
13	-	+ 0.025 mM Zn(C2H3O2)	Escherichia coli
21	-	+ 0.025 mM CoSO4, additional incubation with 0.025 mM Fe2+	Escherichia coli
26	-	+ 0.025 mM CoSO4	Escherichia coli
27	-	-	Escherichia coli
45	-	+ 0.025 mM MnSO4	Escherichia coli
62	-	+ 0.025 mM MnSO4, additional incubation with 0.025 mM Fe2+	Escherichia coli
65	-	+ 0.025 mM FeSO4, additional incubation with 0.025 mM Fe2+	Escherichia coli
87	-	+ 0.025 mM FeSO4	Escherichia coli
87	-	+ 0.025 mM CrCl2, additional incubation with 0.025 mM Fe2+	Escherichia coli
87	-	apoCDase, additional incubation with 0.025 mM Fe2+	Escherichia coli
88	-	+ 0.025 mM CaCl2, additional incubation with 0.025 mM Fe2+	Escherichia coli
94	-	+ 0.025 SnCl2, additional incubation with 0.025 mM Fe2+	Escherichia coli

Storage Stability	Organism
5°C, loses 40% of its activity after 2 weeks, lost activity can be restored by treatment with 0.05 mM FeSO4	Escherichia coli

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-fluorocytosine + H2O	-	Escherichia coli	5-fluorouracil + NH3	-	?
cytosine + H2O	-	Escherichia coli	uracil + NH3	-	?

Subunits	Comment	Organism
tetramer	4 * 48000, SDS-PAGE	Escherichia coli

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
73	-	extremely thermostable	Escherichia coli