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Literature summary for 3.5.4.1 extracted from

  • Yu, T.; Sakai, T.; Omata, S.
    Kinetic properties of cytosine deaminase from Pseudomonas aureofaciens (1976), Agric. Biol. Chem., 40, 551-557.
No PubMed abstract available
Search for more literature for 3.5.4.1

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 1 mM, relative activity 108% Pseudomonas chlororaphis subsp. aureofaciens
alpha,alpha'-dipyridyl 1 mM, relative activity 104% Pseudomonas chlororaphis subsp. aureofaciens
ATP
-
 Serratia marcescens
ATP 1.0 mM, substrate cytosine, relative activity 105%, substrate 5-methylcytosine relative activity 113% Pseudomonas chlororaphis subsp. aureofaciens
dGMP 1.0 mM, substrate cytosine, relative activity 109% Pseudomonas chlororaphis subsp. aureofaciens
EDTA 1 mM, relative activity 111% Pseudomonas chlororaphis subsp. aureofaciens
ITP
-
 Serratia marcescens
ITP
-
 Pseudomonas chlororaphis subsp. aureofaciens
L-amino acids
-
 Serratia marcescens
L-amino acids
-
 Pseudomonas chlororaphis subsp. aureofaciens
L-asparagine
-
 Serratia marcescens
L-asparagine 10.0 mM, relative activity 156% Pseudomonas chlororaphis subsp. aureofaciens
L-aspartic acid
-
 Serratia marcescens
L-aspartic acid 10.0 mM, relative activity 158% Pseudomonas chlororaphis subsp. aureofaciens
L-cysteine 10.0 mM, relative activity 138% Pseudomonas chlororaphis subsp. aureofaciens
L-Glutamic acid 10.0 mM, relative activity 135% Pseudomonas chlororaphis subsp. aureofaciens
L-glutamine 10.0 mM, relative activity 120% Pseudomonas chlororaphis subsp. aureofaciens
L-histidine
-
 Serratia marcescens
L-histidine 10.0 mM, relative activity 148% Pseudomonas chlororaphis subsp. aureofaciens
L-hydroxyproline 10.0 mM, relative activity 141% Pseudomonas chlororaphis subsp. aureofaciens
L-methionine
-
 Serratia marcescens
L-methionine 10.0 mM, relative activity 139% Pseudomonas chlororaphis subsp. aureofaciens
NaCN 1 mM, relative activity 104% Pseudomonas chlororaphis subsp. aureofaciens
phosphate
-
 Serratia marcescens
phosphate 1.0 mM, relative activity 140% Pseudomonas chlororaphis subsp. aureofaciens
Sodium azide 1 mM, relative activity 101% Pseudomonas chlororaphis subsp. aureofaciens
trichloroacetate 1 mM, relative activity 101% Pseudomonas chlororaphis subsp. aureofaciens

Inhibitors

Inhibitors Comment Organism Structure
deoxyguanosine
-
 Serratia marcescens
Mn2+
-
 Pseudomonas chlororaphis subsp. aureofaciens
p-mercuribenzoate
-
 Pseudomonas chlororaphis subsp. aureofaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.57
-
 5-Methylcytosine
-
 Pseudomonas chlororaphis subsp. aureofaciens
4.5
-
 cytosine
-
 Pseudomonas chlororaphis subsp. aureofaciens

Metals/Ions

Metals/Ions Comment Organism Structure
BaCl2 1.0 mM, relative activity 105% Pseudomonas chlororaphis subsp. aureofaciens
CaCl2 1.0 mM, relative activity 113% Pseudomonas chlororaphis subsp. aureofaciens
FeCl3
-
 Serratia marcescens
FeCl3 1.0 mM, relative activity 130% Pseudomonas chlororaphis subsp. aureofaciens
KCl 1.0 mM, relative activity 116% Pseudomonas chlororaphis subsp. aureofaciens
NaCl 1.0 mM, relative activity 107% Pseudomonas chlororaphis subsp. aureofaciens
SnCl2 1.0 mM, relative activity 122% Pseudomonas chlororaphis subsp. aureofaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
580000
-
-
 Serratia marcescens
630000
-
-
 Pseudomonas chlororaphis subsp. aureofaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cytosine + H2O Serratia marcescens
-
 uracil + NH3
-
 ?
cytosine + H2O Pseudomonas chlororaphis subsp. aureofaciens
-
 uracil + NH3
-
 ?
cytosine + H2O Serratia marcescens type S
-
 uracil + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas chlororaphis subsp. aureofaciens
-
 type P
-
Serratia marcescens
-
 type S
-
Serratia marcescens type S
-
 type S
-

Oxidation Stability

Oxidation Stability Organism
stable Serratia marcescens
unstable Pseudomonas chlororaphis subsp. aureofaciens

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas chlororaphis subsp. aureofaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-methylcytosine + H2O
-
 Pseudomonas chlororaphis subsp. aureofaciens thymine + NH3
-
 ?
cytosine + H2O
-
 Serratia marcescens uracil + NH3
-
 ?
cytosine + H2O
-
 Pseudomonas chlororaphis subsp. aureofaciens uracil + NH3
-
 ?
cytosine + H2O
-
 Serratia marcescens type S uracil + NH3
-
 ?

Subunits

Subunits Comment Organism
octamer
-
 Serratia marcescens
polymer
-
 Pseudomonas chlororaphis subsp. aureofaciens

Synonyms

Synonyms Comment Organism
cytosine deaminase P
-
 Pseudomonas chlororaphis subsp. aureofaciens
cytosine deaminase S
-
 Serratia marcescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
 Pseudomonas chlororaphis subsp. aureofaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Pseudomonas chlororaphis subsp. aureofaciens