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Literature summary for 3.5.4.1 extracted from
- Purification of cytosine deaminase from Pseudomonas aureofaciens (1975), Agric. Biol. Chem., 39, 2015-2020.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| unstable in absence of reducing agents | Pseudomonas chlororaphis subsp. aureofaciens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
- |
Saccharomyces cerevisiae |
| 45000 | - |
12-16 * 45000, SDS-PAGE | Pseudomonas chlororaphis subsp. aureofaciens |
| 72000 | - |
8 * 72000, SDS-PAGE | Serratia marcescens |
| 580000 | - |
gel filtration | Serratia marcescens |
| 630000 | - |
gel filtration | Pseudomonas chlororaphis subsp. aureofaciens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cytosine + H2O | Alcaligenes faecalis | - |
uracil + NH3 | - |
? |  |
| cytosine + H2O | Bacillus subtilis | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Escherichia coli | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Saccharomyces cerevisiae | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Enterobacter cloacae | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Pseudomonas fluorescens | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Klebsiella aerogenes | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Pseudomonas aeruginosa | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Pseudomonas putida | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Serratia marcescens | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Proteus mirabilis | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Proteus vulgaris | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Pseudomonas schuylkilliensis | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Pseudomonas taetrolens | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Pseudomonas fragi | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Citrobacter freundii | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Pseudomonas chlororaphis | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Pseudomonas trifolii | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Pseudomonas cruciviae | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Micrococcus flavus | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Ralstonia solanacearum | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Pseudomonas chlororaphis subsp. aureofaciens | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Serratia polymuthicum | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Pseudomonas coronafaciens pv. striafaciens | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Alcaligenes faecalis type S | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Serratia marcescens type S | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Enterobacter cloacae IAM 1221 | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Pseudomonas schuylkilliensis type S | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Escherichia coli K12 AKU 0005 | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Pseudomonas chlororaphis type S | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Proteus vulgaris type S | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Pseudomonas aeruginosa type S | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Pseudomonas putida type S | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Proteus mirabilis type S | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Pseudomonas trifolii type S | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Enterobacter cloacae type S | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Alcaligenes faecalis AKU 0101 | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Serratia polymuthicum AKU 0062 | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Pseudomonas cruciviae type S | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Micrococcus flavus AKU 0502 | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Klebsiella aerogenes type S | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Ralstonia solanacearum type S | - |
uracil + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alcaligenes faecalis | - |
AKU 0101 | - |
| Alcaligenes faecalis | - |
type S | - |
| Alcaligenes faecalis AKU 0101 | - |
AKU 0101 | - |
| Alcaligenes faecalis type S | - |
type S | - |
| Bacillus subtilis | - |
IFO 3026 | - |
| Citrobacter freundii | - |
K-1 AKU 0011 | - |
| Citrobacter freundii | - |
S-96 AKU 0009 | - |
| Enterobacter cloacae | - |
type S | - |
| Enterobacter cloacae | - |
IAM 1221 | - |
| Enterobacter cloacae IAM 1221 | - |
IAM 1221 | - |
| Enterobacter cloacae type S | - |
type S | - |
| Escherichia coli | - |
- |
- |
| Escherichia coli K12 AKU 0005 | - |
- |
- |
| Klebsiella aerogenes | - |
type S | - |
| Klebsiella aerogenes | - |
IFO 3319 | - |
| Klebsiella aerogenes type S | - |
type S | - |
| Micrococcus flavus | - |
AKU 0502 | - |
| Micrococcus flavus AKU 0502 | - |
AKU 0502 | - |
| Proteus mirabilis | - |
type S | - |
| Proteus mirabilis | - |
IFO 3849 | - |
| Proteus mirabilis type S | - |
type S | - |
| Proteus vulgaris | - |
type S | - |
| Proteus vulgaris | - |
IFO 3851 | - |
| Proteus vulgaris type S | - |
type S | - |
| Pseudomonas aeruginosa | - |
type S | - |
| Pseudomonas aeruginosa | - |
IFO 3456 | - |
| Pseudomonas aeruginosa type S | - |
type S | - |
| Pseudomonas chlororaphis | - |
type S | - |
| Pseudomonas chlororaphis | - |
IFO 3904 | - |
| Pseudomonas chlororaphis subsp. aureofaciens | - |
type P | - |
| Pseudomonas chlororaphis subsp. aureofaciens | - |
IFO 3521 | - |
| Pseudomonas chlororaphis type S | - |
type S | - |
| Pseudomonas coronafaciens pv. striafaciens | - |
IFO 3309 | - |
| Pseudomonas cruciviae | - |
type S | - |
| Pseudomonas cruciviae | - |
IFO 12047 | - |
| Pseudomonas cruciviae type S | - |
type S | - |
| Pseudomonas fluorescens | - |
IFO 3081 | - |
| Pseudomonas fluorescens | - |
type P | - |
| Pseudomonas fragi | - |
IFO 3458 | - |
| Pseudomonas putida | - |
type S | - |
| Pseudomonas putida | - |
IFO 3757 | - |
| Pseudomonas putida | - |
IFO 3738 | - |
| Pseudomonas putida type S | - |
type S | - |
| Pseudomonas schuylkilliensis | - |
type S | - |
| Pseudomonas schuylkilliensis | - |
IFO 12055 | - |
| Pseudomonas schuylkilliensis type S | - |
type S | - |
| Pseudomonas taetrolens | - |
IFO 3460 | - |
| Pseudomonas trifolii | - |
type S | - |
| Pseudomonas trifolii | - |
IFO 12056 | - |
| Pseudomonas trifolii type S | - |
type S | - |
| Ralstonia solanacearum | - |
type S | - |
| Ralstonia solanacearum | - |
IFO 12510 | - |
| Ralstonia solanacearum type S | - |
type S | - |
| Saccharomyces cerevisiae | - |
baker's yeast | - |
| Serratia marcescens | - |
type S | - |
| Serratia marcescens | - |
IFO 3054 | - |
| Serratia marcescens type S | - |
type S | - |
| Serratia polymuthicum | - |
AKU 0062 | - |
| Serratia polymuthicum AKU 0062 | - |
AKU 0062 | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| stable | Serratia marcescens |
| unstable | Pseudomonas chlororaphis subsp. aureofaciens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Serratia marcescens |
- |
Pseudomonas chlororaphis subsp. aureofaciens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.1 | - |
- |
Bacillus subtilis |
| 0.1 | - |
- |
Enterobacter cloacae |
| 0.1 | - |
- |
Pseudomonas putida |
| 0.1 | - |
- |
Pseudomonas taetrolens |
| 0.1 | - |
- |
Pseudomonas fragi |
| 0.1 | - |
- |
Micrococcus flavus |
| 0.1 | - |
- |
Serratia polymuthicum |
| 0.1 | - |
- |
Pseudomonas coronafaciens pv. striafaciens |
| 0.14 | - |
- |
Proteus vulgaris |
| 0.19 | - |
- |
Pseudomonas fluorescens |
| 0.22 | - |
- |
Pseudomonas schuylkilliensis |
| 0.22 | - |
- |
Pseudomonas trifolii |
| 0.23 | - |
- |
Pseudomonas putida |
| 0.26 | - |
strain K-1 AKU 0011 | Citrobacter freundii |
| 0.31 | - |
- |
Pseudomonas coronafaciens pv. striafaciens |
| 0.32 | - |
strain AKU 0009 | Citrobacter freundii |
| 0.41 | - |
- |
Pseudomonas cruciviae |
| 0.44 | - |
- |
Serratia marcescens |
| 0.52 | - |
- |
Klebsiella aerogenes |
| 0.58 | - |
- |
Pseudomonas chlororaphis subsp. aureofaciens |
| 0.6 | - |
- |
Pseudomonas aeruginosa |
| 0.62 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-methylcytosine + H2O | - |
Saccharomyces cerevisiae | thymine + NH3 | - |
? | |
| 5-methylcytosine + H2O | - |
Pseudomonas chlororaphis subsp. aureofaciens | thymine + NH3 | - |
? | |
| cytosine + H2O | - |
Alcaligenes faecalis | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Bacillus subtilis | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Escherichia coli | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Saccharomyces cerevisiae | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Enterobacter cloacae | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Pseudomonas fluorescens | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Klebsiella aerogenes | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Pseudomonas aeruginosa | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Pseudomonas putida | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Serratia marcescens | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Proteus mirabilis | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Proteus vulgaris | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Pseudomonas schuylkilliensis | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Pseudomonas taetrolens | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Pseudomonas fragi | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Citrobacter freundii | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Pseudomonas chlororaphis | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Pseudomonas trifolii | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Pseudomonas cruciviae | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Micrococcus flavus | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Ralstonia solanacearum | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Pseudomonas chlororaphis subsp. aureofaciens | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Serratia polymuthicum | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Pseudomonas coronafaciens pv. striafaciens | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Alcaligenes faecalis type S | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Serratia marcescens type S | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Enterobacter cloacae IAM 1221 | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Pseudomonas schuylkilliensis type S | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Escherichia coli K12 AKU 0005 | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Pseudomonas chlororaphis type S | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Proteus vulgaris type S | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Pseudomonas aeruginosa type S | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Pseudomonas putida type S | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Proteus mirabilis type S | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Pseudomonas trifolii type S | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Enterobacter cloacae type S | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Alcaligenes faecalis AKU 0101 | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Serratia polymuthicum AKU 0062 | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Pseudomonas cruciviae type S | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Micrococcus flavus AKU 0502 | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Klebsiella aerogenes type S | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Ralstonia solanacearum type S | uracil + NH3 | - |
? | |
| additional information | - |
Pseudomonas putida | ? | - |
? | |
| additional information | 5-methylcytosine, cytidine, 5'-CMP, adenine, adenosine, 5'-AMP, guanine, guanosine, 5'-GMP and GMP are no substrates | Serratia marcescens | ? | - |
? | |
| additional information | 5-methylcytosine, cytidine, 5'-CMP, adenine, adenosine, 5'-AMP, guanine, guanosine, 5'-GMP and GMP are no substrates | Serratia marcescens type S | ? | - |
? | |
| additional information | - |
Pseudomonas putida type S | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 72000, SDS-PAGE | Serratia marcescens |
| polymer | 12-16 * 45000, SDS-PAGE | Pseudomonas chlororaphis subsp. aureofaciens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
75% activity remains after treatment for 10 min, pH 7 | Serratia marcescens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Serratia marcescens |
| 8 | 9 | - |
Pseudomonas chlororaphis subsp. aureofaciens |
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