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Literature summary for 3.5.3.9 extracted from

  • Xu, Z.W.; Zhou, H.X.; Huang, W.N.
    Some properties of the allantoate amidohydrolase from French bean seedlings (2004), Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao, 30, 460-468.
    View publication on PubMed
Search for more literature for 3.5.3.9

Activating Compound

Activating Compound Comment Organism Structure
lysine
-
 Phaseolus vulgaris
additional information phenylhydrazine crucial for enzyme activity Phaseolus vulgaris
tryptophan
-
 Phaseolus vulgaris

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 0.15 mM completely inhibits incubation mixture with Mn2+ Phaseolus vulgaris
Cu2+ 0.15 mM completely inhibits incubation mixture with Mn2+ Phaseolus vulgaris
EDTA abolishes activation of enzyme by Mn2+; final concentration of 2 mM Phaseolus vulgaris
Fe2+ 0.15 mM completely inhibits incubation mixture with Mn2+ Phaseolus vulgaris
glycolate 50% activity loss with 0.06 mM glycolate for 10 min at 30°C; strong inhibitor, 50% inhibition at 0.06 mM Phaseolus vulgaris
glyoxylate 50% activity loss with 0.02 mM glycolate for 10 min at 30°C; strong inhibitor, 50% inhibition at 0.02 mM Phaseolus vulgaris
guanidine 10% and 80% activity loss with 2 M and 5 M L-guanidine pre-treatment for 10 min at 30°C Phaseolus vulgaris
iminourea pre-treatment with 2mM or 5mM results in a loss of acitivity of 10% or 80% of activity Phaseolus vulgaris
additional information pre-treatment with N-acetylimidazole, iodoacetic acid or 5,5'-dithiobis-(2-nitrobenzoic acid) has no effect on enzyme activity Phaseolus vulgaris
N-Acetylimidazole 5.6% activity loss with 10 mM n-acetylimidazole 10 min at 30°C Phaseolus vulgaris
N-bromosuccinimide 100% activity loss with 10 mM n-bromosuccinimide for 10 min at 30°C; pre-treatment completely inhibits activity Phaseolus vulgaris
pyridoxal 5'-phosphate pre-treatment with 10 mM completely inhibits activity Phaseolus vulgaris
pyridoxal-5-phosphate 100% activity loss with 10 mM pyridoxal-5-phosphate for 10 min at 30°C Phaseolus vulgaris
SDS 50% activity loss with 50 mM SDS pre-treatment for 10 min at 30°C; pre-treatment with 50 mM results in a loss of acitivity of 50% of activity Phaseolus vulgaris
Urea 5% and 20% activity loss with 2 M and 5 M urea pre-treatment for 10 min at 30°C Phaseolus vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.076
-
 Allantoate
-
 Phaseolus vulgaris
76
-
 Allantoate
-
 Phaseolus vulgaris

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ can replace Mn2+, 85% of activity measured in the presence of Mn2+ Phaseolus vulgaris
Mn2+ no enzyme activity without Mn2+, maximal activity at concentrations between 0.1 and 0.5 mM Phaseolus vulgaris
Mn2+ essential for enzyme activity, maximal activity between 0.1-0.5 mM Phaseolus vulgaris
additional information Mg2+, Ca2+, Cu2+, Co2+ and Fe2+ can not replace Mn2+ Phaseolus vulgaris
Ni2+ can replace Mn2+, 32% of activity measured in the presence of Mn2+ Phaseolus vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13500
-
 gel filtration Phaseolus vulgaris
13500
-
 gel filtration on Sephacryl S-200 column Phaseolus vulgaris

Organism

Organism UniProt Comment Textmining
Phaseolus vulgaris
-
 french bean
-
Phaseolus vulgaris
-
 cv. baishui
-

Purification (Commentary)

Purification (Comment) Organism
579fold purified ond DEAE-Sephadex column, ADE2 pool Phaseolus vulgaris
gel filtration with Sephacryl S-200 and Sephadex A50 columns, and dialysis against Tris-HCl pH 7.5 at 4°C Phaseolus vulgaris

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Phaseolus vulgaris
-
leaf
-
 Phaseolus vulgaris
-
root
-
 Phaseolus vulgaris
-
seed
-
 Phaseolus vulgaris
-
seedling
-
 Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0004
-
 ungerminated seeds Phaseolus vulgaris
0.00138
-
 in crude extract after first purification Phaseolus vulgaris
0.00138
-
 crude extract from seedlings Phaseolus vulgaris
0.0018
-
 6-d-old seedlings Phaseolus vulgaris
0.776
-
 579fold purified enzyme from seedlings Phaseolus vulgaris
0.8
-
 after 579-fold purification Phaseolus vulgaris
1
-
 vmax of partially purified ADE2 pool Phaseolus vulgaris

Storage Stability

Storage Stability Organism
-30°C, 2 months, 5% loss of activity Phaseolus vulgaris
-30°C, Tris-HCl buffer, pH 7.5, 2 months Phaseolus vulgaris
4°C, 1 week, 10% loss of activity Phaseolus vulgaris
4°C, Tris-HCl buffer, pH 7.5, 1 week Phaseolus vulgaris
55°C, 15 min, 15% loss of activity Phaseolus vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
allantoate + 2 H2O
-
 Phaseolus vulgaris ureidoglycolate + CO2 + 2 NH3
-
 ?
allantoate + H2O
-
 Phaseolus vulgaris ureidoglycine + NH3 + CO2
-
 ?

Synonyms

Synonyms Comment Organism
ADE2
-
 Phaseolus vulgaris
ALAH
-
 Phaseolus vulgaris
Allantoate amidinohydrolase
-
 Phaseolus vulgaris
allantoate amidohydrolase
-
 Phaseolus vulgaris
allantoate amidohydrolase ALAH Phaseolus vulgaris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Phaseolus vulgaris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 treatment for 15 min reveals a loss of 15% of acitivity Phaseolus vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Phaseolus vulgaris
8.5
-
 in tricine/NaOH buffer Phaseolus vulgaris