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Literature summary for 3.5.3.8 extracted from
- Purification and properties of formylglutamate amidohydrolase from Pseudomonas putida (1987), J. Bacteriol., 169, 4696-4702.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-acetyl-L-glutamate | competitive inhibitor | Pseudomonas putida |  |
| N-formyl-L-aspartate | competitive inhibitor | Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14 | - |
N-formimidoyl-L-glutamate | - |
Pseudomonas putida | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Pseudomonas putida | |
| Cd2+ | - |
Pseudomonas putida | |
| Co2+ | - |
Pseudomonas putida | |
| Fe2+ | - |
Pseudomonas putida | |
| Mg2+ | - |
Pseudomonas putida | |
| Mn2+ | - |
Pseudomonas putida | |
| Ni2+ | - |
Pseudomonas putida | |
| Zn2+ | - |
Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formimidoyl-L-glutamate + H2O | Pseudomonas putida | - |
L-glutamate + formamide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 100 | - |
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formimidoyl-L-glutamate + H2O | - |
Pseudomonas putida | L-glutamate + formamide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 50000, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Pseudomonas putida |
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Release 2023.1 (January 2023)
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