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Literature summary for 3.5.3.7 extracted from
- An arginine regulated gamma-guanidobutyrate ureahydrolase from tench liver (Tinca tinca L.) (1992), Arch. Int. Physiol. Biochim. Biophys., 100, 55-60.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 5 mM complete inhibition | Tinca tinca |  |
| Zn2+ | 92% inhibition | Tinca tinca | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 45 | - |
4-guanidinobutyric acid | 5 mM arginine added | Tinca tinca | |
| 100 | 200 | L-arginine | - |
Tinca tinca | |
| 230 | - |
4-guanidinobutyric acid | - |
Tinca tinca | |
| 450 | - |
4-guanidopropionic acid | - |
Tinca tinca | |
| 1670 | - |
L-(+)-(S)-canavanine | - |
Tinca tinca | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Pseudomonas fluorescens | |
| Mn2+ | - |
Pseudomonas putida | |
| Mn2+ | - |
Streptomyces griseus | |
| Mn2+ | - |
Helix pomatia | |
| Mn2+ | - |
Penicillium roqueforti | |
| Mn2+ | - |
Gallus sp. | |
| Mn2+ | - |
Iguania | |
| Mn2+ | - |
Raja clavata | |
| Mn2+ | absolute requirement for its arginase activity | Tinca tinca | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
6 * 35000 | Tinca tinca |
| 170000 | - |
- |
Tinca tinca |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-Guanidinobutyrate + H2O | Pseudomonas fluorescens | - |
4-Aminobutyrate + urea | - |
? | |
| 4-Guanidinobutyrate + H2O | Pseudomonas putida | - |
4-Aminobutyrate + urea | - |
? | |
| 4-Guanidinobutyrate + H2O | Streptomyces griseus | - |
4-Aminobutyrate + urea | - |
? | |
| 4-Guanidinobutyrate + H2O | Helix pomatia | - |
4-Aminobutyrate + urea | - |
? | |
| 4-Guanidinobutyrate + H2O | Penicillium roqueforti | - |
4-Aminobutyrate + urea | - |
? | |
| 4-Guanidinobutyrate + H2O | Gallus sp. | - |
4-Aminobutyrate + urea | - |
? | |
| 4-Guanidinobutyrate + H2O | Iguania | - |
4-Aminobutyrate + urea | - |
? | |
| 4-Guanidinobutyrate + H2O | Raja clavata | - |
4-Aminobutyrate + urea | - |
? | |
| 4-Guanidinobutyrate + H2O | Tinca tinca | - |
4-Aminobutyrate + urea | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus sp. | - |
chicken | - |
| Helix pomatia | - |
snail | - |
| Iguania | - |
- |
- |
| Penicillium roqueforti | - |
- |
- |
| Pseudomonas fluorescens | - |
- |
- |
| Pseudomonas putida | - |
P2 (ATCC 25571) | - |
| Raja clavata | - |
ray | - |
| Streptomyces griseus | - |
- |
- |
| Tinca tinca | - |
teleostan fresh-water tench, autochtonous fish, L. | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Tinca tinca |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatopancreas | - |
Helix pomatia | - |
| liver | - |
Gallus sp. | - |
| liver | - |
Iguania | - |
| liver | - |
Raja clavata | - |
| liver | - |
Tinca tinca | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, quite stable, retains basically the same activity several weeks, temperatures below 0°C have negative effects | Tinca tinca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-guanidopropionate + H2O | - |
Tinca tinca | ? | - |
? | |
| 4-Guanidinobutyrate + H2O | - |
Pseudomonas fluorescens | 4-Aminobutyrate + urea | - |
? | |
| 4-Guanidinobutyrate + H2O | - |
Pseudomonas putida | 4-Aminobutyrate + urea | - |
? | |
| 4-Guanidinobutyrate + H2O | - |
Streptomyces griseus | 4-Aminobutyrate + urea | - |
? | |
| 4-Guanidinobutyrate + H2O | - |
Helix pomatia | 4-Aminobutyrate + urea | - |
? | |
| 4-Guanidinobutyrate + H2O | - |
Penicillium roqueforti | 4-Aminobutyrate + urea | - |
? | |
| 4-Guanidinobutyrate + H2O | - |
Gallus sp. | 4-Aminobutyrate + urea | - |
? | |
| 4-Guanidinobutyrate + H2O | - |
Iguania | 4-Aminobutyrate + urea | - |
? | |
| 4-Guanidinobutyrate + H2O | - |
Raja clavata | 4-Aminobutyrate + urea | - |
? | |
| 4-Guanidinobutyrate + H2O | - |
Tinca tinca | 4-Aminobutyrate + urea | - |
? | |
| 4-guanidopropionic acid + H2O | - |
Tinca tinca | 3-aminopropionate + urea | - |
? | |
| arginine + H2O | - |
Tinca tinca | 2,5-diaminopentanoate + urea | - |
? | |
| L-canavanine + H2O | - |
Tinca tinca | L-2-amino-4-(aminooxy)butanoic acid + urea | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 35000 | Tinca tinca |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tench liver isoenzyme II | - |
Tinca tinca |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
no variation up to, higher temperatures have negative effect | Tinca tinca |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Pseudomonas fluorescens |
| 9.5 | - |
- |
Pseudomonas putida |
| 9.5 | - |
- |
Streptomyces griseus |
| 9.5 | - |
- |
Helix pomatia |
| 9.5 | - |
- |
Penicillium roqueforti |
| 9.5 | - |
- |
Gallus sp. |
| 9.5 | - |
- |
Iguania |
| 9.5 | - |
- |
Raja clavata |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | - |
Tinca tinca |
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