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Literature summary for 3.5.3.6 extracted from

  • Patil, M.D.; Rathod, V.P.; Bihade, U.R.; Banerjee, U.C.
    Purification and characterization of arginine deiminase from Pseudomonas putida Structural insights of the differential affinities of L-arginine analogues (2019), J. Biosci. Bioeng., 127, 129-137 .
    View publication on PubMed
Search for more literature for 3.5.3.6

Inhibitors

Inhibitors Comment Organism Structure
3-aminoalanine 10 mM, 3% loss of activity. 200 mM, 28% loss of activity Pseudomonas putida
Ag+
-
 Pseudomonas putida
agmatine 10 mM, 8% loss of activity. 200 mM, 28% loss of activity Pseudomonas putida
Cu2+
-
 Pseudomonas putida
D-arginine 10 mM, 4% loss of activity. 200 mM, 97% loss of activity Pseudomonas putida
guanidine 10 mM, 9% loss of activity. 200 mM, 35% loss of activity Pseudomonas putida
Hg2+
-
 Pseudomonas putida
L-canavanine 10 mM, 45% loss of activity. 60 mM, 99% loss of activity Pseudomonas putida
L-homoarginine 10 mM, 2% loss of activity. 200 mM, 25% loss of activity Pseudomonas putida
L-ornithine 10 mM, 12% loss of activity. 200 mM, 24% loss of activity Pseudomonas putida
putrescine 10 mM, 6% loss of activity. 200 mM, 11% loss of activity Pseudomonas putida
Zn2+
-
 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6
-
 L-arginine pH and temperature not specified in the publication Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arginine + H2O Pseudomonas putida
-
 L-citrulline + NH3
-
 ?
L-arginine + H2O Pseudomonas putida ATCC 47054
-
 L-citrulline + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q88P52
-
-
Pseudomonas putida ATCC 47054 Q88P52
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
70.3
-
 pH and temperature not specified in the publication Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O
-
 Pseudomonas putida L-citrulline + NH3
-
 ?
L-arginine + H2O
-
 Pseudomonas putida ATCC 47054 L-citrulline + NH3
-
 ?

Synonyms

Synonyms Comment Organism
ADI
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Pseudomonas putida

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 45 20°C: about 80% of maximal activity, 45°C: about 80% of maximal activity Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.4 phosphate buffer Pseudomonas putida