Literature summary for 3.5.3.6 extracted from

Ni, Y.; Liu, Y.; Schwaneberg, U.; Zhu, L.; Li, N.; Li, L.; Sun, Z.
Rapid evolution of arginine deiminase for improved anti-tumor activity (2011), Appl. Microbiol. Biotechnol., 90, 193-201.

Search for more literature for 3.5.3.6

Protein Variants

Protein Variants	Comment	Organism
A128T/H404R/I410L	in contrast to wild-type mutant shows very high activity activity at pH 7.4 and pH 6.0, Km (L-Arg) 0.43 at pH 6.0, Km (L-Arg) 0.65 at pH 7.4, pH optimum: 6.5	Pseudomonas plecoglossicida
R18L	mutant shows higher activity than wild-type at pH 7.4 and pH 6.0	Pseudomonas plecoglossicida
V10F	mutant shows higher activity than wild-type at pH 7.4 and pH 6.0	Pseudomonas plecoglossicida

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.88	-	L-Arg	pH 6.0, 37°C, wild-type	Pseudomonas plecoglossicida

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas plecoglossicida	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Arg + H2O	-	Pseudomonas plecoglossicida	L-citrulline + NH3	-	?

Synonyms

Synonyms	Comment	Organism
PpADI	-	Pseudomonas plecoglossicida

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	wild-type	Pseudomonas plecoglossicida
6.5	-	mutant A128T/H404R/I410L	Pseudomonas plecoglossicida
7.4	-	wild-type shows only 10% activity	Pseudomonas plecoglossicida

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Release 2023.1 (January 2023)