Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star(DE3) cells | Giardia intestinalis |
Protein Variants | Comment | Organism |
---|---|---|
C424A | catalytically inactive | Giardia intestinalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2S)-2-amino-5-(N''-cyanocarbamimidamido)pentanoic acid | - |
Giardia intestinalis | |
agmatine | very weak, mixed-type inhibitor | Giardia intestinalis | |
additional information | Nomega-cyano-L-arginine does not inhibit Giardia lamblia arginine deiminase | Giardia intestinalis | |
S-nitroso-L-homocysteine | active site directed, irreversible inhibitor | Giardia intestinalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
Nomega-amino-L-arginine | in 50 mM K+-HEPES (pH 7.5), at 25°C | Giardia intestinalis | |
0.16 | - |
L-arginine | in 50 mM K+-HEPES (pH 7.5), at 25°C | Giardia intestinalis | |
2.1 | - |
L-arginine ethyl ester | in 50 mM K+-HEPES (pH 7.5), at 25°C | Giardia intestinalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64189 | - |
2 * 64189, calculated from amino acid sequence | Giardia intestinalis |
64190 | - |
2 * 64190, ESI-MS analysis | Giardia intestinalis |
127000 | - |
gel filtration | Giardia intestinalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Giardia intestinalis | Q27657 | - |
- |
Giardia intestinalis isolate WB, clone 1267 | Q27657 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography and butyl-Sepharose column chromatography | Giardia intestinalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | - |
Giardia intestinalis | L-citrulline + NH3 | - |
? | |
L-arginine + H2O | - |
Giardia intestinalis isolate WB, clone 1267 | L-citrulline + NH3 | - |
? | |
L-arginine ethyl ester + H2O | - |
Giardia intestinalis | ? | - |
? | |
L-arginine ethyl ester + H2O | - |
Giardia intestinalis isolate WB, clone 1267 | ? | - |
? | |
additional information | does not accept agmatine, L-homoarginine, Nalpha-benzoyl-L-arginine ethyl ester as substrate | Giardia intestinalis | ? | - |
? | |
additional information | does not accept agmatine, L-homoarginine, Nalpha-benzoyl-L-arginine ethyl ester as substrate | Giardia intestinalis isolate WB, clone 1267 | ? | - |
? | |
Nalpha-benzoyl-L-arginine + H2O | - |
Giardia intestinalis | ? | - |
? | |
Nalpha-benzoyl-L-arginine + H2O | - |
Giardia intestinalis isolate WB, clone 1267 | ? | - |
? | |
Nomega-amino-L-arginine + H2O | - |
Giardia intestinalis | hydrazine + L-citrulline | - |
? | |
Nomega-amino-L-arginine + H2O | - |
Giardia intestinalis isolate WB, clone 1267 | hydrazine + L-citrulline | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 64189, calculated from amino acid sequence | Giardia intestinalis |
homodimer | 2 * 64190, ESI-MS analysis | Giardia intestinalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.58 | - |
Nomega-amino-L-arginine | in 50 mM K+-HEPES (pH 7.5), at 25°C | Giardia intestinalis | |
2.3 | - |
L-arginine ethyl ester | in 50 mM K+-HEPES (pH 7.5), at 25°C | Giardia intestinalis | |
2.5 | - |
Nalpha-benzoyl-L-arginine | in 50 mM K+-HEPES (pH 7.5), at 25°C | Giardia intestinalis | |
2.6 | - |
L-arginine | in 50 mM K+-HEPES (pH 7.5), at 25°C | Giardia intestinalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Giardia intestinalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | the kcat value is maximal from pH 5.5 to pH 9.0 | Giardia intestinalis |
6 | 8 | the kcat_Km value is maximal between pH 6.0 and 8.0 | Giardia intestinalis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
40 | - |
S-nitroso-L-homocysteine | in 50 mM K+-HEPES (pH 7.5), at 25°C | Giardia intestinalis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
Nomega-amino-L-arginine | in 50 mM K+-HEPES (pH 7.5), at 25°C | Giardia intestinalis | |
2.3 | - |
Nalpha-benzoyl-L-arginine | in 50 mM K+-HEPES (pH 7.5), at 25°C | Giardia intestinalis | |
16 | - |
L-arginine | in 50 mM K+-HEPES (pH 7.5), at 25°C | Giardia intestinalis |