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Literature summary for 3.5.3.6 extracted from
- Expression of arginine deiminase from Pseudomonas plecoglossicida CGMCC2039 in Escherichia coli and its anti-tumor activity (2009), Curr. Microbiol., 58, 593-598.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the recombinant enzyme can effectively inhibit H22 tumor growth at a total dose of 5 U/mouse over a 2-week dosing period | Pseudomonas plecoglossicida |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain BL21 with an N-terminal His6-tag | Pseudomonas plecoglossicida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46500 | - |
deduced ADI protein sequence | Pseudomonas plecoglossicida |
| 49000 | - |
SDS-PAGE | Pseudomonas plecoglossicida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas plecoglossicida | A7LKB0 | - |
- |
| Pseudomonas plecoglossicida CGMCC2039 | A7LKB0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel-chelated agarose gel column chromatography | Pseudomonas plecoglossicida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.76 | - |
at pH 6.0 and 37°C | Pseudomonas plecoglossicida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Arg + H2O | - |
Pseudomonas plecoglossicida | citrulline + NH3 | - |
? |  |
| L-Arg + H2O | - |
Pseudomonas plecoglossicida CGMCC2039 | citrulline + NH3 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Pseudomonas plecoglossicida |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | - |
Pseudomonas plecoglossicida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 60 | about 20% relative activity at 5°C, about 35% relative activity at 20°C, about 20% relative activity at 5°C, about 98% relative activity at 53°C, about 40% relative activity at 50°C, about 10% relative activity at 60°C | Pseudomonas plecoglossicida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Pseudomonas plecoglossicida |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | - |
Pseudomonas plecoglossicida |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | about 25% relative activity at pH 4.0, about 50% relative activity at pH 5.0, about 25% relative activity at pH 7.0, less than 10% relative activity at pH 8.0 | Pseudomonas plecoglossicida |
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