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Literature summary for 3.5.3.6 extracted from
- Expression, purification, and characterization of arginine deiminase from Lactococcus lactis ssp. lactis ATCC 7962 in Escherichia coli BL21 (2007), Protein Expr. Purif., 53, 9-15.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene arcA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) | Lactococcus lactis subsp. lactis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.67 | - |
L-arginine | pH 7.2, 60°C | Lactococcus lactis subsp. lactis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
3 * 46000, recombinant enzyme, SDS-PAGE | Lactococcus lactis subsp. lactis |
| 140000 | - |
recombinant enzyme, gel filtration | Lactococcus lactis subsp. lactis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginine + H2O | Lactococcus lactis subsp. lactis | ADI is the key enzyme of the ADI pathway | L-citrulline + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactococcus lactis subsp. lactis | Q2HYA0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21(DE3) by sequential anion exchange chromatography and gel filtration to near homogeneity | Lactococcus lactis subsp. lactis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 140.3 | - |
purified recombinant enzyme, pH 7.2, 37°C | Lactococcus lactis subsp. lactis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginine + H2O | - |
Lactococcus lactis subsp. lactis | L-citrulline + NH3 | - |
? | |
| L-arginine + H2O | ADI is the key enzyme of the ADI pathway | Lactococcus lactis subsp. lactis | L-citrulline + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | MALDI-TOF mass spectrometry, three-dimensional structure determination and anaylsis, structure comparison | Lactococcus lactis subsp. lactis |
| trimer | 3 * 46000, recombinant enzyme, SDS-PAGE | Lactococcus lactis subsp. lactis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADI | - |
Lactococcus lactis subsp. lactis |
| LADI | - |
Lactococcus lactis subsp. lactis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Lactococcus lactis subsp. lactis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Lactococcus lactis subsp. lactis |
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Release 2023.1 (January 2023)
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