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Literature summary for 3.5.3.6 extracted from

  • Galkin, A.; Lu, X.; Dunaway-Mariano, D.; Herzberg, O.
    Crystal structures representing the Michaelis complex and the thiouronium reaction intermediate of Pseudomonas aeruginosa arginine deiminase (2005), J. Biol. Chem., 280, 34080-34087.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
mutants C406A, H287A, D280A, and D166A in complex with L-arginine, protein solutions containing 20 mM arginine are mixed with an equal volume of mother liquor containing 34-38% 2-methyl-2,4-pentanediol, 6.0-7.0% PEG 3350, 0.1 M Tris-HCl, pH 7.6, and 20 mM arginine, equilibration against the mother liquor reservoir, 2-8 weeks, X-ray diffraction structure determination and anaylsis at 2.3-2.9 A resolution, modeling Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
C406A site-directed mutagenesis, the catalytic residue mutant, substrate binding structure, overview Pseudomonas aeruginosa
D166A site-directed mutagenesis, the catalytic residue mutant, substrate binding structure, overview Pseudomonas aeruginosa
D280A site-directed mutagenesis, the catalytic residue mutant, substrate binding structure, overview Pseudomonas aeruginosa
H278A site-directed mutagenesis, the catalytic residue mutant, substrate binding structure, overview Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arginine + H2O Pseudomonas aeruginosa
-
L-citrulline + NH3
-
ir

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa P13981
-
-

Reaction

Reaction Comment Organism Reaction ID
L-arginine + H2O = L-citrulline + NH3 Cys406, His278, Asp280, and Asp166 residues form the catalytic motif, function of Cys406 in nucleophilic catalysis, detailed reaction mechanism and enzyme-intermediate structures, substrate-induced fit in ADI catalysis, overview Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O
-
Pseudomonas aeruginosa L-citrulline + NH3
-
ir
L-arginine + H2O via enzyme Cys-bound S-alkyl-thiouronium intermediate, substrate binding and enzyme-intermediate structure of wild-type and mutant enzymes, modeling, overview Pseudomonas aeruginosa L-citrulline + NH3
-
ir
additional information structural basis for substrate specificity, overview Pseudomonas aeruginosa ?
-
?

Synonyms

Synonyms Comment Organism
ADI
-
Pseudomonas aeruginosa