Crystallization (Comment) | Organism |
---|---|
mutants C406A, H287A, D280A, and D166A in complex with L-arginine, protein solutions containing 20 mM arginine are mixed with an equal volume of mother liquor containing 34-38% 2-methyl-2,4-pentanediol, 6.0-7.0% PEG 3350, 0.1 M Tris-HCl, pH 7.6, and 20 mM arginine, equilibration against the mother liquor reservoir, 2-8 weeks, X-ray diffraction structure determination and anaylsis at 2.3-2.9 A resolution, modeling | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
C406A | site-directed mutagenesis, the catalytic residue mutant, substrate binding structure, overview | Pseudomonas aeruginosa |
D166A | site-directed mutagenesis, the catalytic residue mutant, substrate binding structure, overview | Pseudomonas aeruginosa |
D280A | site-directed mutagenesis, the catalytic residue mutant, substrate binding structure, overview | Pseudomonas aeruginosa |
H278A | site-directed mutagenesis, the catalytic residue mutant, substrate binding structure, overview | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | Pseudomonas aeruginosa | - |
L-citrulline + NH3 | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | P13981 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-arginine + H2O = L-citrulline + NH3 | Cys406, His278, Asp280, and Asp166 residues form the catalytic motif, function of Cys406 in nucleophilic catalysis, detailed reaction mechanism and enzyme-intermediate structures, substrate-induced fit in ADI catalysis, overview | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | - |
Pseudomonas aeruginosa | L-citrulline + NH3 | - |
ir | |
L-arginine + H2O | via enzyme Cys-bound S-alkyl-thiouronium intermediate, substrate binding and enzyme-intermediate structure of wild-type and mutant enzymes, modeling, overview | Pseudomonas aeruginosa | L-citrulline + NH3 | - |
ir | |
additional information | structural basis for substrate specificity, overview | Pseudomonas aeruginosa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADI | - |
Pseudomonas aeruginosa |