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Literature summary for 3.5.3.24 extracted from
- Dual biosynthesis pathway for longer-chain polyamines in the hyperthermophilic archaeon Thermococcus kodakarensis (2010), J. Bacteriol., 192, 4991-5001.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermococcus kodakarensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00642 | - |
N1-aminopropylagmatine | pH 7.5, 70°C | Thermococcus kodakarensis |  |
| 0.486 | - |
agmatine | pH 7.5, 70°C | Thermococcus kodakarensis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N1-aminopropylagmatine + H2O | Thermococcus kodakarensis | in Pyrococcus kodakaraensis spermidine is synthesized mainly from agmatine. The enzyme is involved in the biosynthesis of spermidine from agmatine | spermidine + urea | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus kodakarensis | Q5JI38 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermococcus kodakarensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agmatine + H2O | 43fold higher kcat/Km value for N1-aminopropylagmatine than for agmatine | Thermococcus kodakarensis | putrescine + urea | - |
? | |
| N1-aminopropylagmatine + H2O | in Pyrococcus kodakaraensis spermidine is synthesized mainly from agmatine. The enzyme is involved in the biosynthesis of spermidine from agmatine | Thermococcus kodakarensis | spermidine + urea | - |
? | |
| N1-aminopropylagmatine + H2O | 43fold higher kcat/Km value for N1-aminopropylagmatine than for agmatine | Thermococcus kodakarensis | spermidine + urea | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TK0882 protein | - |
Thermococcus kodakarensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
assay at | Thermococcus kodakarensis |
| 90 | - |
- |
Thermococcus kodakarensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
enzyme is not fully unfolded | Thermococcus kodakarensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
N1-aminopropylagmatine | pH 7.5, 70°C | Thermococcus kodakarensis | |
| 0.00186 | - |
agmatine | pH 7.5, 70°C | Thermococcus kodakarensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Thermococcus kodakarensis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00387 | - |
agmatine | pH 7.5, 70°C | Thermococcus kodakarensis | |
| 0.165 | - |
N1-aminopropylagmatine | pH 7.5, 70°C | Thermococcus kodakarensis | |
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Release 2023.1 (January 2023)
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