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Literature summary for 3.5.3.21 extracted from
- Purification and characterization of an enzyme from a strain of Ochrobactrum anthropi that degrades condensation products of urea and formaldehyde (ureaform) (1997), Can. J. Microbiol., 43, 1111-1117.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Acetohydroxamate | - |
Brucella anthropi |  |
| Borate | - |
Brucella anthropi | |
| CdSO4 | - |
Brucella anthropi | |
| CuSO4 | - |
Brucella anthropi | |
| HgCl2 | - |
Brucella anthropi | |
| NiCl2 | - |
Brucella anthropi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
4 * 38000, SDS-PAGE | Brucella anthropi |
| 125000 | - |
native PAGE | Brucella anthropi |
| 140000 | - |
gel filtration | Brucella anthropi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brucella anthropi | - |
- |
- |
| Brucella anthropi UF4 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Brucella anthropi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 64.29 | - |
- |
Brucella anthropi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Allantoate + H2O | at 5% of the activity with methylenediurea | Brucella anthropi | Glyoxylate + urea + ammonium | - |
? | |
| Allantoate + H2O | at 5% of the activity with methylenediurea | Brucella anthropi UF4 | Glyoxylate + urea + ammonium | - |
? | |
| dimethylenetriurea + H2O | - |
Brucella anthropi | ammonium + formaldehyde + urea + CO2 | ammonium:formaldehyde:urea at a molar ratio of 4:2:1 | ? | |
| dimethylenetriurea + H2O | - |
Brucella anthropi UF4 | ammonium + formaldehyde + urea + CO2 | ammonium:formaldehyde:urea at a molar ratio of 4:2:1 | ? | |
| methylenediurea + H2O | - |
Brucella anthropi | ammonium + formaldehyde + urea + CO2 | ammonium:formaldehyde:urea at a molar ratio of 2:1:1 | ? | |
| methylenediurea + H2O | - |
Brucella anthropi UF4 | ammonium + formaldehyde + urea + CO2 | ammonium:formaldehyde:urea at a molar ratio of 2:1:1 | ? | |
| trimethylenetetraurea + H2O | - |
Brucella anthropi | ammonium + formaldehyde + urea + CO2 | ammonium:formaldehyde:urea at a molar ratio of 6:3:1 | ? | |
| trimethylenetetraurea + H2O | - |
Brucella anthropi UF4 | ammonium + formaldehyde + urea + CO2 | ammonium:formaldehyde:urea at a molar ratio of 6:3:1 | ? | |
| Ureidoglycolate + H2O | at 130% of the activity with methylenediurea | Brucella anthropi | Glyoxylate + urea | - |
? | |
| Ureidoglycolate + H2O | at 130% of the activity with methylenediurea | Brucella anthropi UF4 | Glyoxylate + urea | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 38000, SDS-PAGE | Brucella anthropi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Brucella anthropi |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | 30°C: about 45% of maximal activity, 70°C: about 30% of maximal activity | Brucella anthropi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
30 min, stable up to | Brucella anthropi |
| 80 | - |
30 min, complete loss of activity | Brucella anthropi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Brucella anthropi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | pH 7: about 25% of maximal activity, pH 9.5: about 70% of maximal activity | Brucella anthropi |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | 60 min, stable | Brucella anthropi |
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