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Literature summary for 3.5.3.20 extracted from
- Diguanidinobutanase of Pseudomonas putida and enzymatic preparation of agmatine from 1,4-diguanidinobutanate (1995), Appl. Microbiol. Biotechnol., 44, 94-99.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | preparation of pure agmatine sulfate | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-Diaminodecane | 41% inhibition at concentration of 3.0 mM | Pseudomonas putida |  |
| 1,7-Diaminoheptane | 22% inhibition at concentration of 5.0 mM | Pseudomonas putida | |
| 1,8-diaminooctane | 34% inhibition at concentration of 3.0 mM | Pseudomonas putida | |
| 1,9-diaminononane | 38% inhibition at concentration of 3.0 mM | Pseudomonas putida | |
| 1-amino-5-guanidinopentane | 29% inhibition at concentration of 5.0 mM | Pseudomonas putida | |
| 5-Guanidinovalerate | 10% inhibition at concentration of 5.0 mM | Pseudomonas putida | |
| agmatine | 28% inhibition at concentration of 5.0 mM | Pseudomonas putida | |
| DTNB | 11% inhibition at concentration of 5.0 mM | Pseudomonas putida | |
| N-carbamoylagmatine | 62% inhibition at concentration of 5.0 mM | Pseudomonas putida | |
| p-chloromercuribenzoate | complete loss of activity at concentration of 5.0 mM, 30% reactivation by incubation with 10.0 mM 2-mercaptoethanol | Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
1,8-Diguanidinooctane | - |
Pseudomonas putida | |
| 0.24 | - |
1,7-Diguanidinoheptane | - |
Pseudomonas putida | |
| 0.33 | - |
1,6-Diguanidinohexane | - |
Pseudomonas putida | |
| 0.5 | - |
1,5-Diguanidinopentane | - |
Pseudomonas putida | |
| 0.65 | - |
1,4-Diguanidinobutane | - |
Pseudomonas putida | |
| 0.91 | - |
1,3-diguanidinopropane | - |
Pseudomonas putida | |
| 1 | - |
1-amino-7-guanidinoheptane | - |
Pseudomonas putida | |
| 1.3 | - |
1-amino-6-guanidinohexane | - |
Pseudomonas putida | |
| 2.5 | - |
1-amino-5-guanidinopentane | - |
Pseudomonas putida | |
| 3 | - |
agmatine | - |
Pseudomonas putida | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inert | Pseudomonas putida | |
| Co2+ | required for full activity | Pseudomonas putida | |
| Mg2+ | inert | Pseudomonas putida | |
| Mn2+ | required for full activity, no inactivation by incubation with 10 mM EDTA | Pseudomonas putida | |
| Mn2+ | two ions per subunit | Pseudomonas putida | |
| Ni2+ | required for full activity | Pseudomonas putida | |
| Zn2+ | inert | Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 170000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,4-diguanidinobutane + H2O | Pseudomonas putida | - |
agmatine + urea | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| full purification | Pseudomonas putida |
Storage Stability
| Storage Stability | Organism |
|---|---|
| pH 7.0-8.0 | Pseudomonas putida |
| pH 7.0-9.0 | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,3-diguanidinopropane + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 1,4-diguanidinobutane + H2O | - |
Pseudomonas putida | agmatine + urea | - |
? | |
| 1,5-diguanidinopentane + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 1,6-diguanidinohexane + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 1,7-diguanidinoheptane + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 1,8-diguanidinooctane + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 1-amino-5-guanidinopentane + H2O | - |
Pseudomonas putida | 1,5-diaminopentane + urea | - |
? | |
| 1-amino-6-guanidinohexane + H2O | - |
Pseudomonas putida | 1,6-diaminohexane + urea | - |
? | |
| 1-amino-7-guanidinoheptane + H2O | - |
Pseudomonas putida | 1,7-diaminoheptane + urea | - |
? | |
| agmatine + H2O | low activity towards agmatine | Pseudomonas putida | putrescine + urea | - |
? | |
| diguanidinoalkanoate + H2O | utilization of alkanoates with 3-10 methylen groups | Pseudomonas putida | aminoalkylguanidine + urea | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4*38000 Da, SDS-PAGE | Pseudomonas putida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
no loss of activity within 2 h in presence of 10 mM EDTA | Pseudomonas putida |
| 40 | - |
stable on heating in presence of Mn2+ | Pseudomonas putida |
| 60 | - |
complete inactivation in presence and absence of Mn2+ | Pseudomonas putida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1330 | - |
1,4-Diguanidinobutane | per subunit | Pseudomonas putida | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | 11 | in sodium carbonate and piperazine hydrochloric buffers | Pseudomonas putida |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | stable in TRIS-HCl | Pseudomonas putida |
| 7 | 8 | stable in potassium phosphate | Pseudomonas putida |
| 10 | - |
stable in piperazine hydrochloride | Pseudomonas putida |
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