Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C274A | no detectable activity | Homo sapiens |
C275A | retains a kcat value about half of wild type protein | Homo sapiens |
H173A | no detectable activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | insensitive to hydrogen peroxide | Homo sapiens | |
S-nitroso-L-homocysteine | - |
Homo sapiens | |
Zn2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33440 | - |
electrospray ionization mass spectrometry | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity resin chromatography and phenyl-Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | L-arginine is no substrate | Homo sapiens | ? | - |
? | |
NG,NG-dimethyl-L-arginine + H2O | - |
Homo sapiens | dimethylamine + L-citrulline | - |
? | |
NG-monomethyl-L-arginine + H2O | - |
Homo sapiens | citrulline + methylamine | - |
? | |
S-methyl-L-thiocitrulline + H2O | - |
Homo sapiens | methanethiol + L-citrulline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DDAH-1 | isozyme | Homo sapiens |
dimethylarginine dimethylaminohydrolase | - |
Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
10 | - |
activity decreases at pH values above 10 | Homo sapiens |