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Literature summary for 3.5.3.18 extracted from

  • Forbes, S.P.; Druhan, L.J.; Guzman, J.E.; Parinandi, N.; Zhang, L.; Green-Church, K.B.; Cardounel, A.J.
    Mechanism of 4-HNE mediated inhibition of hDDAH-1: implications in no regulation (2008), Biochemistry, 47, 1819-1826.
    View publication on PubMed
Search for more literature for 3.5.3.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21star cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxy-nonenal dose-dependently inhibits DDAH activity with 15% inhibition at 0.01 mM and complete inhibition at 0.5 mM Homo sapiens
H2O2 43% inhibition at 1 mM Homo sapiens
additional information exposure to pathophysiological levels of reactive oxygen has little to no effect on the activity of the enzyme Homo sapiens
OH radical 47% inhibition at 1 mM Homo sapiens
peroxynitrite inhibition of 15% after exposure to peroxynitrite at concentrations ranging from 0.01-0.1 mM, 25% inhibition at 1 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.536
-
 NG-methyl-L-arginine at 37°C Homo sapiens
0.687
-
 NG,NG-dimethyl-L-arginine at 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ Zn2+-containing enzyme Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 DDAH-1, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and HiTrap Q Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information incapable of hydrolyzing peptide incorporated methylarginines Homo sapiens ?
-
 ?
NG,NG-dimethyl-L-arginine + H2O
-
 Homo sapiens dimethylamine + L-citrulline
-
 ?
NG-methyl arginine + H2O
-
 Homo sapiens L-citrulline + methylamine
-
 ?

Synonyms

Synonyms Comment Organism
DDAH-1
-
 Homo sapiens
dimethylarginine dimethylamino-hydrolase
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.05
-
 at 37°C Homo sapiens 4-hydroxy-nonenal