Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | 5 mM of L-cysteine, L-homocysteine, glutathione, dithiothreitol, or Tris(2-carboxyethyl)phosphine-HCl for 30 min at 37°C cannot restore inhibited activity, suggesting that the covalent product N-thiosulfoximide will remain stable under the reducing conditions present in the cytosol | Bos taurus | |
S-nitroso-L-cysteine | 9-15% inhibition of the zinc-free enzyme at 0.5 mM, S-nitrosylation of the active site Cys273 in DDAH-1 | Bos taurus | |
S-nitroso-L-homocysteine | 96% inhibition of the zinc-free enzyme at 0.5 mM, leads to the unique formation of an N-thiosulfoximide | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | Zn2+ binding to DDAH-1 protects the enzyme from S-nitrosylation by free nitric oxide | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31200 | - |
electrospray ionization mass spectrometry | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P56965 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
kidney | - |
Bos taurus | - |
Synonyms | Comment | Organism |
---|---|---|
DDAH-1 | - |
Bos taurus |
Nomega,Nomega-dimethyl-L-arginine dimethylaminohydrolase-1 | - |
Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.69 | - |
S-nitroso-L-homocysteine | in the presence of 5 mM EDTA, at pH 7.3 and 37°C | Bos taurus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.075 | - |
in 50 mM HEPES/NaOH (pH 7.3), 150 mM KCl, and 5 mM EDTA for 30 min at 37°C | Bos taurus | S-nitroso-L-homocysteine |