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Literature summary for 3.5.3.18 extracted from

  • Braun, O.; Knipp, M.; Chesnov, S.; Vasak, M.
    Specific reactions of S-nitrosothiols with cysteine hydrolases: A comparative study between dimethylargininase-1 and CTP synthetase (2007), Protein Sci., 16, 1522-1534.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
additional information 5 mM of L-cysteine, L-homocysteine, glutathione, dithiothreitol, or Tris(2-carboxyethyl)phosphine-HCl for 30 min at 37°C cannot restore inhibited activity, suggesting that the covalent product N-thiosulfoximide will remain stable under the reducing conditions present in the cytosol Bos taurus
S-nitroso-L-cysteine 9-15% inhibition of the zinc-free enzyme at 0.5 mM, S-nitrosylation of the active site Cys273 in DDAH-1 Bos taurus
S-nitroso-L-homocysteine 96% inhibition of the zinc-free enzyme at 0.5 mM, leads to the unique formation of an N-thiosulfoximide Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ Zn2+ binding to DDAH-1 protects the enzyme from S-nitrosylation by free nitric oxide Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31200
-
electrospray ionization mass spectrometry Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P56965
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Bos taurus
-
kidney
-
Bos taurus
-

Synonyms

Synonyms Comment Organism
DDAH-1
-
Bos taurus
Nomega,Nomega-dimethyl-L-arginine dimethylaminohydrolase-1
-
Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.69
-
S-nitroso-L-homocysteine in the presence of 5 mM EDTA, at pH 7.3 and 37°C Bos taurus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.075
-
in 50 mM HEPES/NaOH (pH 7.3), 150 mM KCl, and 5 mM EDTA for 30 min at 37°C Bos taurus S-nitroso-L-homocysteine