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Literature summary for 3.5.3.18 extracted from

  • Plevin, M.J.; Magalhaes, B.S.; Harris, R.; Sankar, A.; Perkins, S.J.; Driscoll, P.C.
    Characterization and manipulation of the Pseudomonas aeruginosa dimethylarginine dimethylaminohydrolase monomer--dimer equilibrium (2004), J. Mol. Biol., 341, 171-184.
    View publication on PubMed

Application

Application Comment Organism
additional information dimerization is not critical for the maintenance of the biological function of the protein Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
E33H weakening of the homodimer interaction observed for wild type protein, expressed in Escherichia coli at levels similar to the wild type Pseudomonas aeruginosa
E33Q weakening of the homodimer interaction observed for wild type protein, expressed in Escherichia coli at levels similar to the wild type Pseudomonas aeruginosa
N36D expressed in Escherichia coli at levels similar to the wild type Pseudomonas aeruginosa
N36H weakening of the homodimer interaction observed for wild type protein, expressed in Escherichia coli at levels similar to the wild type Pseudomonas aeruginosa
N36W more stable homodimer than the wild type protein, expressed in Escherichia coli at levels similar to the wild type Pseudomonas aeruginosa
Q43H weakening of the homodimer interaction observed for wild type protein, expressed in Escherichia coli at levels similar to the wild type Pseudomonas aeruginosa
Q43R weakening of the homodimer interaction observed for wild type protein, expressed in Escherichia coli at levels similar to the wild type Pseudomonas aeruginosa
R40E weakening of the homodimer interaction observed for wild type protein, expressed in Escherichia coli at levels similar to the wild type Pseudomonas aeruginosa
R40E-R98H exclusively monomeric, expressed in Escherichia coli at levels similar to the wild type Pseudomonas aeruginosa
R40E/R98H about 95% of wild type activity, stable monomer Pseudomonas aeruginosa
R40W not expressed in Escherichia coli at levels similar to the wild type Pseudomonas aeruginosa
R98H weakening of the homodimer interaction observed for wild type protein, expressed in Escherichia coli at levels similar to the wild type Pseudomonas aeruginosa
R98N not expressed in Escherichia coli at levels similar to the wild type Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
monomer, hydrodynamic measurement Pseudomonas aeruginosa
58000
-
dimer, hydrodynamic measurement Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I4E3
-
-

Purification (Commentary)

Purification (Comment) Organism
by immobilized nickel-affinity chromatography and gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Nomega-monomethyl-L-arginine + H2O
-
Pseudomonas aeruginosa L-citrulline + methylamine
-
?

Subunits

Subunits Comment Organism
dimer 2 * 29000, dynamic equilibrium between monomer and homodimer, hydrodynamic measurements Pseudomonas aeruginosa
homodimer PaDDAH exists in dynamic equilibrium between symmetric homodimer and monomer states Pseudomonas aeruginosa
monomer 1 * 29000, dynamic equilibrium between monomer and homodimer, hydrodynamic measurements Pseudomonas aeruginosa
monomer PaDDAH exists in dynamic equilibrium between monomer and symmetric homodimer states Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
dimethylarginine dimethylaminohydrolase
-
Pseudomonas aeruginosa
PaDDAH
-
Pseudomonas aeruginosa