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Literature summary for 3.5.3.18 extracted from
- Structural and functional characterization of the Zn(II) site in dimethylargininase-1 (DDAH-1) from bovine brain. Zn(II) release activates DDAH-1 (2001), J. Biol. Chem., 276, 40449-40456.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-histidine | activating ability in decreasing order: histamine, imidazole, L-histidine, D-histidine | Bos taurus |  |
| histamine | activating ability in decreasing order: histamine, imidazole, L-histidine, D-histidine | Bos taurus | |
| imidazole | activating ability in decreasing order: histamine, imidazole, L-histidine, D-histidine | Bos taurus | |
| L-histidine | activating ability in decreasing order: histamine, imidazole, L-histidine, D-histidine | Bos taurus | |
| additional information | type of buffer dramatically influences enzyme activity, activity in imidazole/HCl buffer is about 25% higher than in phosphate buffer | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methylamine | 10 mM, 80% of activity | Bos taurus | |
| Zn2+ | endogenous inhibitor, regulatory role | Bos taurus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| method for reversible preparation of metal-free and metal-containing enzyme | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.55 | - |
37°C | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N,N-dimethyl-L-arginine + H2O | - |
Bos taurus | dimethylamine + L-citrulline | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DDAH-1 | - |
Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
type of buffer dramatically influences enzymatic activity | Bos taurus |
| 6.2 | - |
phosphate buffer | Bos taurus |
| 6.8 | - |
imidazole/HCl buffer | Bos taurus |
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Release 2023.1 (January 2023)
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