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Literature summary for 3.5.3.18 extracted from

  • Ogawa, T.; Kimoto, M.; Sasaoka, K.
    Purification and properties of a new enzyme, NG,NG-dimethylarginine dimethylaminohydrolase from rat kidney (1989), J. Biol. Chem., 264, 10205-10209.
    View publication on PubMed
Search for more literature for 3.5.3.18

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
 Rattus norvegicus
Cu2+
-
 Rattus norvegicus
HgCl2
-
 Rattus norvegicus
L-citrulline competitive Rattus norvegicus
NEM
-
 Rattus norvegicus
PCMB
-
 Rattus norvegicus
Zn2+
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 NG,NG-dimethyl-L-arginine
-
 Rattus norvegicus
0.36
-
 NG-monomethyl-L-arginine
-
 Rattus norvegicus
9.24
-
 NG,NG-dimethyl-L-arginine
-
 Rattus norvegicus
33
-
 NG,NG-Dimethyl-L-homoarginine
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 gel filtration Rattus norvegicus
34000
-
 1 * 34000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NG,NG-dimethyl-L-arginine + H2O Rattus norvegicus enzyme plays a role in metabolism of NG,NG-dimethyl-L-arginine dimethylamine + L-citrulline
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
kidney
-
 Rattus norvegicus
-
liver
-
 Rattus norvegicus
-
lung
-
 Rattus norvegicus
-
pancreas
-
 Rattus norvegicus
-
skeletal muscle
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-30°C, 0.1 M sodium phosphate buffer, pH 7.0, 10% glycerol, 1 mM 2-mercaptoethanol Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NG,NG-dimethyl-L-arginine + H2O
-
 Rattus norvegicus dimethylamine + L-citrulline
-
 ir
NG,NG-dimethyl-L-arginine + H2O enzyme plays a role in metabolism of NG,NG-dimethyl-L-arginine Rattus norvegicus dimethylamine + L-citrulline
-
 ?
NG,NG-dimethyl-L-homoarginine + H2O at 2.8% of the activity with NG,NG-dimethyl-L-arginine Rattus norvegicus ?
-
 ?
NG-monomethyl-L-arginine + H2O at 61% of the activity with NG,NG-dimethyl-L-arginine Rattus norvegicus citrulline + methylamine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 34000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
-
 Rattus norvegicus
45
-
 stable Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.009
-
 NG,NG-Dimethyl-L-homoarginine
-
 Rattus norvegicus
0.094
-
 NG-monomethyl-L-arginine
-
 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 hydrolysis of NG,NG-dimethyl-L-arginine Rattus norvegicus
6.7
-
 hydrolysis of NG-monomethyl-L-arginine Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8.5 stable between Rattus norvegicus