Crystallization (Comment) | Organism |
---|---|
in complex with inhibitor Cl-amidine, to 1.6 A resolution | Porphyromonas gingivalis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | strain T2 shows three amino-acid substitutions directly preceding catalytic residue H236 (G231N/E232T/N235D) when compared with PPAD from the reference strain ATCC 33277. Mutations localize to a loop engaged in substrate/inhibitor binding. Mutation of these positions in the reference strain results in twofold higher cell-associated citrullinating activity. Similar to PPAD-T1, recombinant PPAD-T2 citrullinates arginines at the C-termini of general peptidic substrates but not within peptides | Porphyromonas gingivalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cl-amidine | inhibitor binds covalently to the catalytic C351-Sgamma atom, mimicking a reaction intermediate | Porphyromonas gingivalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0054 | - |
PPGFSPFR | pH 7.5, 37°C | Porphyromonas gingivalis | |
0.0074 | - |
IHAREIFDSR | pH 7.5, 37°C | Porphyromonas gingivalis | |
0.038 | - |
IHAREIFDSR | pH 7.5, 37°C | Porphyromonas gingivalis | |
0.045 | - |
PPGFSPFR | pH 7.5, 37°C | Porphyromonas gingivalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Porphyromonas gingivalis | - |
- |
- |
Porphyromonas gingivalis | Q9RQJ2 | - |
- |
Porphyromonas gingivalis ATCC 33277 | - |
- |
- |
Porphyromonas gingivalis T2 | Q9RQJ2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
IHAREIFDSR + H2O | peptide derived from enolase | Porphyromonas gingivalis | IHAREIFDS-citrulline + NH3 | - |
? | |
IHAREIFDSR + H2O | peptide derived from enolase | Porphyromonas gingivalis ATCC 33277 | IHAREIFDS-citrulline + NH3 | - |
? | |
IHAREIFDSR + H2O | peptide derived from enolase | Porphyromonas gingivalis T2 | IHAREIFDS-citrulline + NH3 | - |
? | |
additional information | no substrate: peptide GFSPFRSS | Porphyromonas gingivalis | ? | - |
? | |
additional information | no substrate: peptide GFSPFRSS | Porphyromonas gingivalis ATCC 33277 | ? | - |
? | |
additional information | no substrate: peptide GFSPFRSS | Porphyromonas gingivalis T2 | ? | - |
? | |
PPGFSPFR + H2O | peptide derived from bradykinin | Porphyromonas gingivalis | PPGFSPF-citrulline + NH3 | - |
? | |
PPGFSPFR + H2O | peptide derived from bradykinin | Porphyromonas gingivalis ATCC 33277 | PPGFSPF-citrulline + NH3 | - |
? | |
PPGFSPFR + H2O | peptide derived from bradykinin | Porphyromonas gingivalis T2 | PPGFSPF-citrulline + NH3 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
67 | - |
melting temperature | Porphyromonas gingivalis |
68 | - |
melting temperature | Porphyromonas gingivalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.88 | - |
IHAREIFDSR | pH 7.5, 37°C | Porphyromonas gingivalis | |
3.03 | - |
PPGFSPFR | pH 7.5, 37°C | Porphyromonas gingivalis | |
7 | - |
IHAREIFDSR | pH 7.5, 37°C | Porphyromonas gingivalis | |
11.3 | - |
PPGFSPFR | pH 7.5, 37°C | Porphyromonas gingivalis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.184 | - |
IHAREIFDSR | pH 7.5, 37°C | Porphyromonas gingivalis | |
0.255 | - |
PPGFSPFR | pH 7.5, 37°C | Porphyromonas gingivalis | |
0.391 | - |
IHAREIFDSR | pH 7.5, 37°C | Porphyromonas gingivalis | |
0.563 | - |
PPGFSPFR | pH 7.5, 37°C | Porphyromonas gingivalis |