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Literature summary for 3.5.3.15 extracted from

  • Rodriguez, S.B.; Stitt, B.L.; Ash, D.E.
    Cysteine 351 is an essential nucleophile in catalysis by Porphyromonas gingivalis peptidylarginine deiminase (2010), Arch. Biochem. Biophys., 504, 190-196.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Porphyromonas gingivalis

Protein Variants

Protein Variants Comment Organism
C351S the mutant is catalytically inactive, exhibiting less than 0.01% wild type activity Porphyromonas gingivalis

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide PAD exhibits pseudo first-order loss of activity in the presence of 0.1-10 mM iodoacetamide. When substrate (0.8 mM N-alpha-benzoyl-L-arginine) is present in the inactivation reaction, PAD retains 88% activity after a 4 min incubation, compared to only 32% residual activity under the same reaction conditions in the absence of substrate Porphyromonas gingivalis
iodoacetate PAD exhibits pseudo first-order loss of activity in the presence of 0.5-20 mM iodoacetate Porphyromonas gingivalis
L-canavanine suicide substrate Porphyromonas gingivalis
additional information substrate protects the enzyme from inactivation Porphyromonas gingivalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60880
-
 calculated mass of truncated PAD with its poly-His and Xpress epitope tags Porphyromonas gingivalis
60890
-
 MALDI-TOF mass spectrometry Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein L-Arg + H2O Porphyromonas gingivalis PAD catalyzes the deimination of the guanidino group from peptidylarginine residues of various peptides to produce peptidylcitrulline and ammonia protein L-citrulline + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-alpha-benzoyl-L-arginine + H2O
-
 Porphyromonas gingivalis N-alpha-benzoyl-L-citrulline + NH3
-
 ?
protein L-Arg + H2O PAD catalyzes the deimination of the guanidino group from peptidylarginine residues of various peptides to produce peptidylcitrulline and ammonia Porphyromonas gingivalis protein L-citrulline + NH3
-
 ?

Synonyms

Synonyms Comment Organism
PAD
-
 Porphyromonas gingivalis
peptidylarginine deiminase
-
 Porphyromonas gingivalis