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Literature summary for 3.5.3.15 extracted from

  • McGraw, W.T.; Potempa, J.; Farley, D.; Travis, J.
    Purification, characterization, and sequence analysis of a potential virulence factor from Porphyromonas gingivalis. Peptidylarginine deiminase (1999), Infect. Immun., 67, 3248-3256.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.15

General Stability

General Stability Organism
FMN or FAD stabilize Porphyromonas gingivalis

Inhibitors

Inhibitors Comment Organism Structure
antipain 8 mM, 41% inhibition Porphyromonas gingivalis
Cys 25 mM, complete inhibition Porphyromonas gingivalis
leupeptin 5 mM, complete inhibition Porphyromonas gingivalis
Nalpha-L-Arg methyl ester 12.5 mM, 48% inhibition Porphyromonas gingivalis
thiocitrulline 12.5 mM, complete inhibition Porphyromonas gingivalis
Thiourea 50 mM, complete inhibition Porphyromonas gingivalis
tosyl-L-leucinechloromethyl ketone 12.5 mM, 98% inhibition Porphyromonas gingivalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0029
-
 benzoyl-L-Arg
-
 Porphyromonas gingivalis
0.028
-
 benzoyl-Gly-L-Arg
-
 Porphyromonas gingivalis
0.047
-
 bradykinin
-
 Porphyromonas gingivalis
0.141
-
 L-Arg
-
 Porphyromonas gingivalis
0.152
-
 benzoyl-L-Arg-NH2
-
 Porphyromonas gingivalis
0.874
-
 benzoyl-L-Arg ethyl ester
-
 Porphyromonas gingivalis

Localization

Localization Comment Organism GeneOntology No. Textmining
vesicular fraction 90% of the deiminase activity is cell- or membrane vesicle-associated Porphyromonas gingivalis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46600
-
 1 * 46600, SDS-PAGE Porphyromonas gingivalis
47000
-
 gel filtration Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein L-Arg + H2O Porphyromonas gingivalis peptidylarginine deiminase, acting in concert with arginine-specific proteinases from Porphyromonas gingivalis, promotes the growth of the pathogen in the periodontal pocket, initially by enhancing its survivability and then by assisting the organism in its circumvention of host humoral defenses ?
-
 ?

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Porphyromonas gingivalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0334
-
-
 Porphyromonas gingivalis

Storage Stability

Storage Stability Organism
-20°C or -80°C, in presence of the stabilizing factors FMN or FAD, at pH 5.5, 7.5 or 9.0, no appreciable loss of activity Porphyromonas gingivalis
4°C, appreciable loss of activity after overnight storage Porphyromonas gingivalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-Arg ethyl ester + H2O
-
 Porphyromonas gingivalis benzoyl-citrulline ethyl ester + NH3
-
 ?
benzoyl-Gly-L-Arg + H2O
-
 Porphyromonas gingivalis benzoyl-Gly-L-citrulline + NH3
-
 ?
benzoyl-L-Arg + H2O
-
 Porphyromonas gingivalis benzoyl-L-citrulline + NH3
-
 ?
benzoyl-L-Arg-NH2 + H2O
-
 Porphyromonas gingivalis benzoyl-L-citrullineamide + NH3
-
 ?
bradykinin + H2O
-
 Porphyromonas gingivalis 9-citrulline bradykinin + NH3
-
 ?
L-Arg + H2O
-
 Porphyromonas gingivalis L-citrulline + NH3
-
 ?
additional information the enzyme undergoes automodification Porphyromonas gingivalis ?
-
 ?
protein L-Arg + H2O bradykinin Porphyromonas gingivalis protein L-citrulline + NH3
-
 ?
protein L-Arg + H2O peptidylarginine deiminase, acting in concert with arginine-specific proteinases from Porphyromonas gingivalis, promotes the growth of the pathogen in the periodontal pocket, initially by enhancing its survivability and then by assisting the organism in its circumvention of host humoral defenses Porphyromonas gingivalis ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 46600, SDS-PAGE Porphyromonas gingivalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Porphyromonas gingivalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.3
-
-
 Porphyromonas gingivalis

pH Range

pH Minimum pH Maximum Comment Organism
7 11 pH 7.0: about 45% of maximal activity, pH 11.0: about 40% of maximal activity Porphyromonas gingivalis