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Literature summary for 3.5.3.14 extracted from

  • Yorifuji, T.; Furuyoshi, S.
    Mn2+ dependent amidinoaspartase in Pseudomonas putida (1986), Agric. Biol. Chem., 50, 1327-1328.
No PubMed abstract available
Search for more literature for 3.5.3.14

Application

Application Comment Organism
medicine test for N-amidino-L-aspartate in the urine of uremic patients Pseudomonas chlororaphis

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Pseudomonas chlororaphis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0094
-
 N-amidino-L-aspartate C-32, activation with Mn2+ Pseudomonas putida
0.21
-
 N-amidino-L-aspartate C-32 , activation with Co2+ Pseudomonas putida
10.3
-
 N-amidino-L-aspartate C-32 Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
 Pseudomonas chlororaphis
Co2+ C-32, 27% of the activation with Mn2+ Pseudomonas putida
Mn2+ C-32, specifically activation Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39800
-
 5 * 39800, C-32, SDS-PAGE Pseudomonas putida
240000
-
 C-32, gel filtration Pseudomonas putida
300000
-
-
 Pseudomonas chlororaphis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-amidino-L-aspartic acid Pseudomonas putida
-
 L-aspartic acid + urea
-
 ?
N-amidino-L-aspartic acid Pseudomonas chlororaphis
-
 L-aspartic acid + urea
-
 ?
N-amidino-L-aspartic acid Pseudomonas putida C-32
-
 L-aspartic acid + urea
-
 ?

Organism

Organism UniProt Comment Textmining
no activity in Pseudomonas chlororaphis
-
-
-
no activity in Pseudomonas chlororaphis ATCC 9446
-
-
-
Pseudomonas chlororaphis
-
 fluorescent Pseudomonas
-
Pseudomonas putida
-
 C-32
-
Pseudomonas putida C-32
-
 C-32
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas chlororaphis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27.9
-
-
 Pseudomonas chlororaphis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme is specific for the L-isomer Pseudomonas chlororaphis ?
-
 ?
additional information C-32, guanidinoacetate, 3-guanidinopropionate, 4-guanidinobutyrate, 5-guanidinovalerate, 6-guanidinocaproate, 2-guanidinoethylsulfate, taurocyamine, 4-guanidino-3-hydroxybutyrate, L-arginine, DL-N-acetylarginine, DL-2-guanidinoisobutyrate, N-amidinoalanine, methylguanidine, guanidine and creatine are no substrates Pseudomonas putida ?
-
 ?
additional information C-32, guanidinoacetate, 3-guanidinopropionate, 4-guanidinobutyrate, 5-guanidinovalerate, 6-guanidinocaproate, 2-guanidinoethylsulfate, taurocyamine, 4-guanidino-3-hydroxybutyrate, L-arginine, DL-N-acetylarginine, DL-2-guanidinoisobutyrate, N-amidinoalanine, methylguanidine, guanidine and creatine are no substrates Pseudomonas putida C-32 ?
-
 ?
N-amidino-L-aspartic acid
-
 Pseudomonas putida L-aspartic acid + urea
-
 ?
N-amidino-L-aspartic acid
-
 Pseudomonas chlororaphis L-aspartic acid + urea
-
 ?
N-amidino-L-aspartic acid
-
 Pseudomonas putida C-32 L-aspartic acid + urea
-
 ?
N-amidino-L-aspartic acid + H2O
-
 Pseudomonas putida L-aspartic acid + urea
-
 ?
N-amidino-L-aspartic acid + H2O
-
 Pseudomonas chlororaphis L-aspartic acid + urea
-
 ?
N-amidino-L-aspartic acid + H2O
-
 Pseudomonas putida C-32 L-aspartic acid + urea
-
 ?

Subunits

Subunits Comment Organism
pentamer 5 * 39800, C-32, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
Mn2+-dependent amidinoaspartase
-
 Pseudomonas chlororaphis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 10 C-32 Pseudomonas putida