Protein Variants | Comment | Organism |
---|---|---|
E235A | lower activity compared to the wild type enzyme | Pseudomonas aeruginosa |
E235Q | lower activity compared to the wild type enzyme | Pseudomonas aeruginosa |
H269A | about 5fold lower activity compared to the wild type enzyme | Pseudomonas aeruginosa |
H269C | about 5fold lower activity compared to the wild type enzyme | Pseudomonas aeruginosa |
H269N | about 5fold lower activity compared to the wild type enzyme | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dipicolinate | zinc can be removed by dialysis against the metal chelator dipicolinate with the complete loss of catalytic activity | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
N-formimino-L-glutamate | wild type enzyme, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
0.66 | - |
N-formimino-L-glutamate | mutant enzyme E235D, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
1.2 | - |
N-formimino-L-glutamate | Cu2+-reconstituted wild type enzyme, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
2.1 | - |
N-formimino-L-glutamate | Cd2+-reconstituted wild type enzyme, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
2.2 | - |
N-formimino-L-glutamate | mutant enzyme E235A, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
2.7 | - |
N-formimino-L-glutamate | Ni2+-reconstituted wild type enzyme, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
19 | - |
N-formimino-L-glutamate | mutant enzyme E235Q, at pH 8.0 and 30°C | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | can replace Zn2+ | Pseudomonas aeruginosa | |
Cu2+ | can replace Zn2+ | Pseudomonas aeruginosa | |
Ni2+ | can replace Zn2+ | Pseudomonas aeruginosa | |
Zn2+ | contains 1.2 equivalents of Zn2+ per protein subunit, essential for activity | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
strain PA01 | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, High Load 26/60 Superdex 200 gel filtration, and Resource Q anion exchange column chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formimino-L-glutamate + H2O | - |
Pseudomonas aeruginosa | N-formyl-L-glutamate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HutF | - |
Pseudomonas aeruginosa |
N-formimino-L-glutamate iminohydrolase | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
N-formimino-L-glutamate | mutant enzyme E235D, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
0.1 | - |
N-formimino-L-glutamate | mutant enzyme E235A, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
0.12 | - |
N-formimino-L-glutamate | mutant enzyme E235Q, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
8.5 | - |
N-formimino-L-glutamate | Cu2+-reconstituted wild type enzyme, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
13.2 | - |
N-formimino-L-glutamate | Zn2+-reconstituted wild type enzyme, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
21.3 | - |
N-formimino-L-glutamate | Ni2+-reconstituted wild type enzyme, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
31 | - |
N-formimino-L-glutamate | Cd2+-reconstituted wild type enzyme, at pH 8.0 and 30°C | Pseudomonas aeruginosa |