Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-chloroacetamidine | - |
Helicobacter pylori | |
2-chloroacetamidine | - |
Porphyromonas gingivalis | |
2-chloroacetamidine | - |
Streptococcus mutans | |
iodoacetamide | - |
Helicobacter pylori | |
iodoacetamide | - |
Porphyromonas gingivalis | |
iodoacetamide | - |
Streptococcus mutans | |
N-(4-aminobutyl)-2-chloroethanimidamide | - |
Helicobacter pylori | |
N-(4-aminobutyl)-2-chloroethanimidamide | - |
Porphyromonas gingivalis | |
N-(4-aminobutyl)-2-chloroethanimidamide | - |
Streptococcus mutans | |
N-(4-aminobutyl)-2-fluoroethanimidamide | inactivation proceeds via a multistep mechanism that requires general acid catalysis | Helicobacter pylori | |
N-(4-aminobutyl)-2-fluoroethanimidamide | inactivation proceeds via a multistep mechanism that requires general acid catalysis | Porphyromonas gingivalis | |
N-(4-aminobutyl)-2-fluoroethanimidamide | inactivation proceeds via a multistep mechanism that requires general acid catalysis | Streptococcus mutans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Porphyromonas gingivalis | - |
- |
- |
Streptococcus mutans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the protein is recombinantly expressed, lysed by sonication, and then purified by a combination of anion exchange, heparin affinity, and gel filtration | Helicobacter pylori |
the protein is recombinantly expressed, lysed by sonication, and then purified by a combination of anion exchange, heparin affinity, and gel filtration | Porphyromonas gingivalis |
the protein is recombinantly expressed, lysed by sonication, and then purified by a combination of anion exchange, heparin affinity, and gel filtration | Streptococcus mutans |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
agmatine + H2O = N-carbamoylputrescine + NH3 | like other GME hydrolases the enzyme possesses a high pKa active site Cys, suggesting that the enzyme employs a reverse protonation mechanism | Helicobacter pylori | |
agmatine + H2O = N-carbamoylputrescine + NH3 | like other GME hydrolases the enzyme possesses a high pKa active site Cys, suggesting that the enzyme employs a reverse protonation mechanism | Porphyromonas gingivalis | |
agmatine + H2O = N-carbamoylputrescine + NH3 | like other GME hydrolases the enzyme possesses a high pKa active site Cys, suggesting that the enzyme employs a reverse protonation mechanism | Streptococcus mutans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agmatine + H2O | - |
Helicobacter pylori | N-carbamoylputrescine + NH3 | - |
? | |
agmatine + H2O | - |
Porphyromonas gingivalis | N-carbamoylputrescine + NH3 | - |
? | |
agmatine + H2O | - |
Streptococcus mutans | N-carbamoylputrescine + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
agmatine deiminase | - |
Porphyromonas gingivalis |
agmatine deiminase | - |
Streptococcus mutans |
agmatine deiminaseHpAgD | - |
Helicobacter pylori |
PgAgD | - |
Porphyromonas gingivalis |
SmAgD | - |
Streptococcus mutans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Helicobacter pylori |
37 | - |
assay at | Porphyromonas gingivalis |
37 | - |
assay at | Streptococcus mutans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Helicobacter pylori |
8 | - |
assay at | Porphyromonas gingivalis |
8 | - |
assay at | Streptococcus mutans |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00026 | - |
pH 8.0, 37°C | Streptococcus mutans | N-(4-aminobutyl)-2-chloroethanimidamide | |
0.00027 | - |
pH 8.0, 37°C | Streptococcus mutans | N-(4-aminobutyl)-2-fluoroethanimidamide | |
0.00087 | - |
pH 8.0, 37°C | Helicobacter pylori | N-(4-aminobutyl)-2-chloroethanimidamide | |
0.0068 | - |
pH 8.0, 37°C | Helicobacter pylori | N-(4-aminobutyl)-2-fluoroethanimidamide | |
0.015 | - |
pH 8.0, 37°C | Porphyromonas gingivalis | N-(4-aminobutyl)-2-chloroethanimidamide | |
0.091 | - |
pH 8.0, 37°C | Porphyromonas gingivalis | N-(4-aminobutyl)-2-fluoroethanimidamide |