Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas aeruginosa PA14 |
Protein Variants | Comment | Organism |
---|---|---|
C364G | complete loss of activity | Pseudomonas aeruginosa PA14 |
R3G/R4S | mutation of the twin-arginine translocation tat-sequence at the N-terminus. Mutant is hardly secreted to periplasm | Pseudomonas aeruginosa PA14 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | isoform Agu2A' contains a twin-arginine translocation signal at its N-terminus and is secreted to the periplasm | Pseudomonas aeruginosa PA14 | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39900 | - |
x * 39900, calculated for mature protein | Pseudomonas aeruginosa PA14 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa PA14 | A0A0H2Z743 | isoform agu2A' | - |
Pseudomonas aeruginosa PA14 | A0A0H2Z8C6 | isoform agu2A | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | cleavage of translocation signal | Pseudomonas aeruginosa PA14 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01055 | - |
pH 9.0, 25°C | Pseudomonas aeruginosa PA14 |
0.015 | - |
pH 9.0, 37°C | Pseudomonas aeruginosa PA14 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agmatine + H2O | - |
Pseudomonas aeruginosa PA14 | N-carbamoylputrescine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39900, calculated for mature protein | Pseudomonas aeruginosa PA14 |
Synonyms | Comment | Organism |
---|---|---|
agu2A | - |
Pseudomonas aeruginosa PA14 |
Organism | Comment | Expression |
---|---|---|
Pseudomonas aeruginosa PA14 | presence of an alternate agmatine operon in the Pseudomonas aeruginosa strain PA14 named agu2ABCA' that contains two genes for agmatine deiminases, agu2A and agu2A'. This operon is present in 25% of clinical Pseudomonas aeruginosa isolates. Agmatine induces expression of the operon during the stationary phase of growth and during biofilm growth and agu2ABCA' provides only weak complementation of aguBA, which is induced during log phase | additional information |
Pseudomonas aeruginosa PA14 | presence of an alternate agmatine operon in the Pseudomonas aeruginosa strain PA14 named agu2ABCA that contains two genes for agmatine deiminases, agu2A and agu2A'. This operon is present in 25% of clinical Pseudomonas aeruginosa isolates. Agmatine induces expression of the operon during the stationary phase of growth and during biofilm growth and agu2ABCA' provides only weak complementation of aguBA, which is induced during log phase | up |
General Information | Comment | Organism |
---|---|---|
physiological function | deletion of the agmatine operon agu2ABCA' reduces biofilm production of strain PA14 following addition of exogenous agmatine | Pseudomonas aeruginosa PA14 |
physiological function | deletion of the agmatine operon agu2ABCA', specifically its secreted product Agu2A', reduces biofilm production of strain PA14 following addition of exogenous agmatine | Pseudomonas aeruginosa PA14 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.136 | - |
agmatine | pH 9.0, 25°C | Pseudomonas aeruginosa PA14 | |
2.624 | - |
agmatine | pH 9.0, 37°C | Pseudomonas aeruginosa PA14 |