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Literature summary for 3.5.3.12 extracted from

  • Llacer, J.L.; Polo, L.M.; Tavarez, S.; Alarcon, B.; Hilario, R.; Rubio, V.
    The gene cluster for agmatine catabolism of Enterococcus faecalis: study of recombinant putrescine transcarbamylase and agmatine deiminase and a snapshot of agmatine deiminase catalyzing its reaction (2007), J. Bacteriol., 189, 1254-1265.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Enterococcus faecalis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method with in 50 mM Tris-HCl, pH 7.45, 1 mM dithiothreitol and 20 mM NaCl Enterococcus faecalis

Inhibitors

Inhibitors Comment Organism Structure
arcaine competitive inhibitor Enterococcus faecalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 SDS-PAGE Enterococcus faecalis

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis Q837U5 strain SD10
-
Enterococcus faecalis ATCC 700802 Q837U5 strain SD10
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap Ni-affinity column chromatography and Superdex 200 HR gel filtration Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agmatine + H2O highly specific for agmatine Enterococcus faecalis N-carbamoylputrescine + NH3
-
 ?
agmatine + H2O highly specific for agmatine Enterococcus faecalis ATCC 700802 N-carbamoylputrescine + NH3
-
 ?
additional information does not use L-arginine, L-argininamide, or arcaine (1,4-diguanidinobutane) as substrates Enterococcus faecalis ?
-
 ?
additional information does not use L-arginine, L-argininamide, or arcaine (1,4-diguanidinobutane) as substrates Enterococcus faecalis ATCC 700802 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer X-ray crystallography and gel filtration Enterococcus faecalis

Synonyms

Synonyms Comment Organism
AgDI
-
 Enterococcus faecalis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.028
-
 arcaine at 37°C Enterococcus faecalis