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Literature summary for 3.5.3.12 extracted from
- Enzymic conversion of agmatine to putrescine in Lathyrus sativus seedlings. Purification and properties of a multifunctional enzyme (putrescine synthase) (1981), J. Biol. Chem., 256, 9532-9541.
General Stability
| General Stability | Organism |
|---|---|
| highly unstable even in presence of glycerol, dithiothreitol and Mg2+, freeze-thawing and dialysis lead to considerable loss of activity | Lathyrus sativus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
gel filtration, PAGE | Lathyrus sativus |
| 56000 | - |
gel filtration, Sephadex G-200 | Lathyrus sativus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agmatine + H2O | Lathyrus sativus | - |
N-carbamoylputrescine + NH3 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lathyrus sativus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lathyrus sativus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Lathyrus sativus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.1 | - |
- |
Lathyrus sativus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C purified enzyme lost all component activities within 48 h | Lathyrus sativus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agmatine + H2O | - |
Lathyrus sativus | N-carbamoylputrescine + NH3 | - |
? | |
| agmatine + H2O | multifunctional enzyme, acts also as putrescine transcarbamylase, putrescine synthase, ornithine transcarbamylase and carbamate kinase | Lathyrus sativus | N-carbamoylputrescine + NH3 | - |
? | |
| agmatine + ornithine + H2O + phosphate | - |
Lathyrus sativus | putrescine + citrulline + NH3 + phosphate | - |
? | |
| carbamoyl phosphate + ADP + H2O | - |
Lathyrus sativus | ATP + CO2 + NH3 | - |
r | |
| carbamoyl phosphate + ornithine | - |
Lathyrus sativus | citrulline + phosphate | - |
r | |
| N-carbamoylputrescine + phosphate | - |
Lathyrus sativus | putrescine + carbamoyl phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 55000, single polypeptide chain, SDS-PAGE | Lathyrus sativus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| putrescine synthase | - |
Lathyrus sativus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
putrescine transcarbamylase and carbamate kinase function | Lathyrus sativus |
| 8 | - |
ornithine transcarbamylase function | Lathyrus sativus |
| 8.8 | - |
agmatine iminohydrolase function | Lathyrus sativus |
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