Cloned (Comment) | Organism |
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residues Ala36-Val352 overexpressed as a fusion with both N- and C-terminal purification tags in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour-diffusion method, agmatinase (residues Ala36-Val352) overexpressed as a fusion with both N- and C-terminal purification tags in Escherichia coli and crystallized in the presence of Mn2+ and 1,6-diaminohexane at 297 K using polyethylene glycol 4000 as a precipitant. X-ray diffraction data are collected at 100 K to 2.49 A from a flash-frozen crystal. The crystals are tetragonal, belonging to space group P4(2), with unit-cell parameters a = b = 114.54 A, c =125.65A, alpha = beta = gamma = 90°. Three monomers are likely to be present in the asymmetric unit, giving a crystal volume per protein weight of 3.66A | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |